| 斑点叉尾鮰病毒对几种淡水鱼类细胞系感染性的初步研究 |
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| 引用本文： | 刘学芹,王敏,王卫民,Yuanan Lu.斑点叉尾鮰病毒对几种淡水鱼类细胞系感染性的初步研究[J].水生态学杂志,2009,30(6):76-79. |
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| 作者姓名： | 刘学芹 王敏 王卫民 Yuanan Lu |
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| 作者单位： | 华中农业大学水产学院 湖北武汉;华中农业大学水产学院 湖北武汉;华中农业大学水产学院 湖北武汉;Department of Public Healith,University of Hawaii,Honolulu,HI 96822,USA |
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| 基金项目： | 教育部博士点新教师基金，湖北省自然科学基金，华中农业大学教师启动基金 |
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| 摘 要： | To study the susceptibility of multiple fresh water fish cell lines to channel catfish virus （CCV） and to evaluate and assess potentially pernicious impact of CCV on several rare and important economic fish species in China. Five cell lines established from several fish species including grass carp fins （GCF）, epithelioma papulosum cyprini （ EPC）, white sturgeon spleen （ WSS - 2 ）, chinook salmon embryo （ CHSE - 214 ） and parental control channel catfish ovary （CCO） were tested and compared for their sensitivity to CCV infection. After virns infection, test cells were characterized by cell morphology, cytopathic effect （CPE） appearance time, and infectious virus titer. Our results indicated that all these cell lines were sensitive to CCV, but at varied levels. Comparative viral challenge assays revealed that, among these five fish cell lines, GCF exhibited to be the most sensitive one to CCV infection, and infectious viral particles produced in this cell line is significantly more than that generated from parental CCO cells. EPC cells were also highly susceptible to CCV infection with a similar level of viral production as compared to CCO. Experimental data showed that all the test five cell lines were susceptible to CCV and present study suggests that CCV can be a potential pathogen to infect gYass carp, Cyprinus carpio and other fresh water fishes.
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| 关 键 词： | 斑点叉尾倒病毒 感染性 细胞系 |
Study on Susceptibility of Multiple Fresh Water Fish Cell Lines to Channel Catfish Virus |
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| LIU Xue-qin,WANG Min,WANG Wei-min.Study on Susceptibility of Multiple Fresh Water Fish Cell Lines to Channel Catfish Virus[J].Journal of Hydroecology,2009,30(6):76-79. |
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| Authors: | LIU Xue-qin WANG Min WANG Wei-min |
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| Institution: | College of Fisheries , Huazhong Agricultural University , Wuhan Hubei 430070, China,College of Fisheries , Huazhong Agricultural University , Wuhan Hubei 430070, China,College of Fisheries , Huazhong Agricultural University , Wuhan Hubei 430070, China |
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| Abstract: | |
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| Keywords: | Channel catfish virus Susceptibility Cell lines |
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