Artificially induced tetraploid masu salmon have the ability to form primordial germ cells |
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| Authors: | Suzu Sakao Takafumi Fujimoto Terumasa Kobayashi Goro Yoshizaki Etsuro Yamaha Katsutoshi Arai |
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| Institution: | (1) Laboratory of Breeding Science, Graduate School of Fisheries Science, Hokkaido University, Hakodate Hokkaido, 041-8611, Japan;(2) Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan;(3) Nanae Fresh Water Laboratory, Field Science Center for Northern Biosphere, Hokkaido University, Kameda Hokkaido, 041-1105, Japan |
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| Abstract: | Diploid gametes generated with tetraploid animals are a stepping stone to improving chromosome manipulation techniques. However,
artificially induced tetraploid individuals generally die soon after hatching. Diploid gametes could be induced by in vivo
cultures of tetraploid primordial germ cells (PGCs) through germ-line chimera. In the present study, characteristics of PGCs
were studied in inviable tetraploid masu salmon, Oncorhynchus masou. Histological observation of tetraploid embryos revealed that the same or smaller numbers of PGCs were observed and they
migrate into the genital ridges as did diploid PGCs during gonadogenesis. By whole-mount in situ hybridization using vasa messenger RNA (mRNA), 4–35 vasa-positive signals were detected in a pair of genital ridges of tetraploids. By cytological
observation of genital ridge cell suspensions, several large round cells were observed, some of which extended pseudopodia.
They also contained large nuclei and round granules in their cytoplasm, characteristics of PGCs. As the results suggest that
inviable artificial tetraploids have PGCs, we expect to achieve diploid gamete production through surrogate propagation and
tetraploid fish production. |
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| Keywords: | Germ-line chimera Primordial germ cells (PGCs) Pseudopodium Surrogate propagation Tetraploidy vasa |
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