Genetic information of three pure lines of Porphyra yezoensis (Bangiales, Rhodophyta) obtained by AFLP analysis |
| |
| Authors: | OSAMU IITSUKA KAYO NAKAMURA AKIYUKI OZAKI NOBUAKI OKAMOTO AND NAOTSUNE SAGA |
| |
| Institution: | ;Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611 and ;Department of Aquatic Biosciences, Tokyo University of Fisheries, Minato, Tokyo 108-8477, Japan |
| |
| Abstract: | Porphyra (Bangiales, Rhodophyta), which includes several valuable marine crops, has recently received great interest as a model plant for fundamental and applied studies in marine sciences. Amplified fragment length polymorphisms (AFLPs) are a robust and efficient means for genetic mapping, linkage analysis of genetic characters for breeding and population studies in land plant genomes. To examine whether AFLPs are applicable as genetic markers in the present study, we detected AFLP markers with three pure lines in order to promote genetic analysis in Porphyra yezoensis . The following five sets of AFLP primer pairs (E-AA, M-CAA) (E-AA, M-CAC) (E-AA, M-CAG) (E-AA, M-CAT) (E-AA, M-CTA) were tested with template DNAs from three pure lines and they showed a total of 227 bands. This suggests that AFLP markers are promising tools for genetic analysis in Porphyra . |
| |
| Keywords: | AFLP genetic marker laver model organism pure line red alga Porphyra yezoensis |
|
|
