| 白斑综合症病毒（WSSV）对中国对虾卵及各期幼体人工感染的试验研究 |
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| 引用本文： | 刘萍,孔杰,李健,阎振广.白斑综合症病毒（WSSV）对中国对虾卵及各期幼体人工感染的试验研究[J].海洋水产研究,2001,22(1):1-6. |
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| 作者姓名： | 刘萍 孔杰 李健 阎振广 |
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| 作者单位： | 1. 中国水产科学研究院黄海水产研究所
2. 青岛海洋大学海洋生命学院 |
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| 基金项目： | 国家“863”项目!(819-0 2 -0 5 )，“973”项目资助! (19990 12 0 0 7) |
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| 摘 要： | 用感染白斑综合症毒病的中国对虾头胸甲，对健康的仔虾进行了人工投喂感染实验，同时从病虾中分离出病毒悬液作为毒种，无节幼体，蚤体 幼体和糠虾体进行不同温度条件下的人工浸浴实验，结果表明，卵和无节幼体，蚤状幼体，糠虾幼对病毒悬液不敏感,闰理切片观察未见病毒粒子，光镜病理切片可观空到鳃上皮，前肠上皮有包涵体样病变，同时在肝胰组织中发现了肝胰腺细小病毒（HPV）包涵体。
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| 关 键 词： | 中国对虾 卵 幼体 白斑综合症病毒 人工感染 病理变化 |
| 修稿时间： | 2000年9月29日 |
Experiment on the ovum and larvae of Penaeus chinensis artificially infected with the white spot syndrome virus(WSSV) |
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| LIU Ping,KONG Jie,LI Jian.Experiment on the ovum and larvae of Penaeus chinensis artificially infected with the white spot syndrome virus(WSSV)[J].Marine Fisheries Research,2001,22(1):1-6. |
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| Authors: | LIU Ping KONG Jie LI Jian |
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| Abstract: | Artificial infection experiment of healthy post-larva of shr impswas conducted by feeding with sick Penaeus chinensis affected by the White spot syndrome virus(WSSV); at the same time, ovum、nauplius、zoea and mysis were soaked in the virus-suspended liquid isolated from the sick shrimps at different temperature. The result showed tha tovum、nauplius、zoea and mysis were not sensitive to the virus-suspended liqu id and there was not feature disease founded in the observation of pathological sections; the observation of sections of post-larva fed with specimen affected by the virus showed that there were lots of viral particles in electronic observ ation. There were inclusion bodys of white spot syndrome virus in the hypodermal tissue of gill and foregut, and there was Hepatopancreatic parvo-like virus (HPV) inclusion bodys by the optic microscope observation. |
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| Keywords: | Ovum and larvae of Penaeus chinensis White spot syndrome virus(WSSV) Artificial infection |
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