| 虾夷扇贝精子形态结构和超低温冷冻损伤的电镜观察 |
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| 引用本文： | 杨培民,杨爱国,刘志鸿,周丽青.虾夷扇贝精子形态结构和超低温冷冻损伤的电镜观察[J].海洋水产研究,2008,29(1):98-102. |
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| 作者姓名： | 杨培民 杨爱国 刘志鸿 周丽青 |
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| 作者单位： | 1. 农业部海洋渔业资源可持续利用重点开放实验室,中国水产科学研究院黄海水产研究所,青岛,266071;上海水产大学生命学院,200090
2. 上海水产大学生命学院,200090 |
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| 基金项目： | 国家科技攻关项目(2004BA526B0103)，青岛市科技发展计划项目(05-1-HY-79)共同资助 |
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| 摘 要： | 应用透射电镜和扫描电镜技术,研究了超低温冷冻保存前后虾夷扇贝精子超微结构和形态的变化。结果显示,虾夷扇贝精子由头部、中段和尾部3部分组成,外被光滑质膜;顶体位于头部最前端,呈倒"v"形,细胞核近似圆柱状,电子密度较高;4个线粒体和两个相互垂直的中心粒构成了精子的中段;鞭毛细长,轴丝为典型的"9 2"结构。经超低温冷冻保存后,冷冻损伤的精子表现为:精子被膜肿胀、被膜与核膜分离、丢失;顶体破裂、内容物流出;线粒体解体、线粒体嵴变形;鞭毛被膜肿胀、部分精子鞭毛脱落。可以推测,超低温冷冻保存对精子膜、顶体、线粒体和鞭毛的损伤可导致冻精活力和受精能力的下降。
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| 关 键 词： | 虾夷扇贝 精子 冷冻损伤 超微结构 |
| 文章编号： | 1000-7075(2008)01-0098-05 |
| 修稿时间： | 2006年9月28日 |
Ultrastructure and cryodamage studies of Patinopecten yessoensis sperm by electron microscopy |
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| YANG Pei-min,YANG Ai-guo,LIU Zhi-hong,ZHOU Li-qing.Ultrastructure and cryodamage studies of Patinopecten yessoensis sperm by electron microscopy[J].Marine Fisheries Research,2008,29(1):98-102. |
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| Authors: | YANG Pei-min YANG Ai-guo LIU Zhi-hong ZHOU Li-qing |
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| Abstract: | Electron microscopy was used to investigate the changes in morphology and ultrastructure of Patinopecten yessoensis(Jay.) sperm after cryopreservation.Fresh sperm was covered by smooth plasma membrane,and was consisted of head,mid-piece and tail.The acrosome took a shape of upside-down V and located at the top of the head.Under the acrosome,the nucleus was column-shaped with a high electron density.Four mitochondria and two orthogonal centrioles formed the mid-piece.The flagellum was thin and long with a typical "9 2" microtubular structure.Cryodamages in cryopreserved and thawed sperm was observed as:the plasma membrane swelled and broke off from the nuclear membrane;the acrosome disrupted and its contents lost;some mitochondria lost external membrane,accompanied by the disaggregation of mitochondria or the deformation of mitochondria cristae;swelling of flagellum membrane or loss of flagellum.Cryodamage on plasma membrane,acrosome,mitochondria and flagellum probably caused the decrease in motility and fertility of the cryopreserved sperm. |
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| Keywords: | Patinopecten yessoensis Spermatozoa Cryodamge Ultrastructure |
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