| 4种海参16SrRNA和COI基因片段序列比较及系统学研究 |
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| 引用本文： | 陈丽梅,李琪,李赟.4种海参16SrRNA和COI基因片段序列比较及系统学研究[J].中国水产科学,2008,15(6). |
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| 作者姓名： | 陈丽梅 李琪 李赟 |
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| 作者单位： | 中国海洋大学,水产学院,山东,青岛,26603 |
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| 基金项目： | 国家科技支撑计划
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| 摘 要： | 采用PCR技术对来自山东荣成、长岛、俄罗斯和日本的刺参(Apostichopus japonicus),来自澳大利亚的黄乳海参(Holothuria fuscogilva),来自冰岛的北大西洋瓜参(Cucumaria frondosa)和来自福建的二色桌片参(Mensamaria interce-dens)的16SrRNA和COI基因片段进行了扩增和测序,分别得到了长度约为500bp和540bp的片段。通过统计变异位点、平均核苷酸差异数和核苷酸多样性指数进行基因序列变异分析。结果表明,根据16SrRNA、COI基因片段进行刺参种内差异比较时,长岛和荣成刺参序列差异最小,和日本刺参序列差异最大;刺参和其他科3种海参种间的序列差异远远高于刺参种内差异。从GenBank中选取7条海参序列与本研究所测序列一同构建了NJ和ME系统树。根据2种基因片段的系统学分析均表明,刺参属和拟刺参属亲缘关系很近,可能有共同的起源。而海参科未与同属于楯手目(Aspidochirolida)的刺参科聚类,而与枝手目瓜参科和沙子鸡科聚为一支,与形态学的分类不一致。
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| 关 键 词： | 海参 16S rRNA基因 COI基因 序列比较 系统发育分析 |
| 修稿时间： | 2010/4/8 0:00:00 |
Sequence analysis of mitochondriai 16S rRNA and COI gene and molecular phylogeny of four species of sea cucumber |
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| CHEN Li-mei,LI Qi,LI Yun.Sequence analysis of mitochondriai 16S rRNA and COI gene and molecular phylogeny of four species of sea cucumber[J].Journal of Fishery Sciences of China,2008,15(6). |
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| Authors: | CHEN Li-mei LI Qi LI Yun |
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| Abstract: | 500 bp 16S rRNA and 540 bp COI fragment were obtained by PCR method from four species of sea cucumber,Apostichopus japonicus,Holothuria fuscogilva,Cucumaria frondosa and Mensamaria intercedens.Variable sites,nucleotide diversity,average number of nucleotide differences and average number of nucleotide substitutions per site were calculated to analyze sequence difference.Phylogenetic trees were constructed by NJ and ME methods.As a result,the biggest intra-species difference was found between individual A.japonicus from Changdao and Japan while the least between those from Changdao and Rongcheng.Differentiation between A.japonicus and other three species was higher than that within A.japonicus.NJ and ME phylogenetic trees based on 16S rRNA and COI gene both show that Apostichopus and Parastichopus were closely related,which indicates the two genera may have a common origin.Holothuriidae doesn't cluster with Stichopodidae but cluster with Cucumariidae and Phyllophoridae,which is different from the result of morphological taxon. |
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| Keywords: | sea cucumber 16S rRNA gene COI gene sequence comparison phylogenetic analysis |
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