| 盐度对罗氏沼虾能量代谢和运动能力的影响 |
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| 引用本文： | 李江涛,邱小龙,邱晓桐,方俊超,黄菲菲,梁日深,李清清,林蠡,吕晓静.盐度对罗氏沼虾能量代谢和运动能力的影响[J].中国水产科学,2021,28(10):1291-1302. |
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| 作者姓名： | 李江涛 邱小龙 邱晓桐 方俊超 黄菲菲 梁日深 李清清 林蠡 吕晓静 |
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| 作者单位： | 仲恺农业工程学院, 广州市水产病害与水禽养殖重点实验室, 广东 广州 510225 |
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| 基金项目： | 国家自然科学基金项目(32002375)； 广东省教育厅青年创新人才项目(2020KQNCX029)； 广东省基础与应用基础研究基金项目(2020A1515410006). |
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| 摘 要： | 为研究盐度对罗氏沼虾(Macrobrachium rosenbergii)能量代谢和运动能力的影响, 设置了 0 (对照)、4、8 和 12 共 4 个盐度胁迫水平, 研究了罗氏沼虾在胁迫 48 h 后的耗氧率、鳃丝和肌肉能量代谢酶活性及其游泳和弹跳能力。结果显示, 耗氧率在盐度升至 4 时并无显著变化(P＞0.05), 但 8 和 12 盐度组的耗氧率显著高于对照组(P＜0.05)。 鳃丝己糖激酶(HK)、磷酸果糖激酶(PFK)和乳酸脱氢酶(LDH)活性在不同盐度条件下并无显著变化(P＞0.05), 但鳃丝丙酮酸脱氢酶(PDH)和细胞色素 C 氧化酶(COX)活性在盐度为 8 时显著升高(P＜0.05), 并且鳃丝 PDH 和 COX 活性均与耗氧率呈显著线性正相关(P＜0.05)。盐度在 8 时罗氏沼虾的游泳足肌肉和腹部肌肉 HK、PFK 和 LDH 活性并无显著变化(P＞0.05), 但游泳足肌肉和腹部肌肉 PDH 和 COX 活性显著下降(P＜0.05); 盐度升至 12 时游泳足肌肉和腹部肌肉 HK、PFK、LDH、PDH 和 COX 活性均显著下降(P＜0.05)。运动能力结果显示, 盐度升至 8 时游泳能力显著下降(P＜0.05), 盐度升至 12 时弹跳能力显著下降(P＜0.05)。游泳能力与游泳足肌肉 PDH 和 COX 活性呈显著线性正相关(P＜0.05); 弹跳能力与腹部肌肉 HK、PFK 和 LDH 活性呈显著线性正相关(P＜0.05)。研究表明, 罗氏沼虾通过增加氧气摄取用于鳃丝有氧代谢以应对盐度胁迫, 但这些生理调节导致肌肉能量代谢酶活性下降, 造成罗氏沼虾的游泳和弹跳能力减弱。
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| 关 键 词： | 盐度 罗氏沼虾 行为 游泳 弹跳 能量代谢 |
Effects of salinity on the energy metabolism and locomotive ability of Macrobrachium rosenbergii |
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| LI Jiangtao,QIU Xiaolong,QIU Xiaotong,FANG Junchao,HUANG Feifei,LIANG Rishen,LI Qingqing,LIN Li,LYU Xiaojing.Effects of salinity on the energy metabolism and locomotive ability of Macrobrachium rosenbergii[J].Journal of Fishery Sciences of China,2021,28(10):1291-1302. |
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| Authors: | LI Jiangtao QIU Xiaolong QIU Xiaotong FANG Junchao HUANG Feifei LIANG Rishen LI Qingqing LIN Li LYU Xiaojing |
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| Institution: | Zhongkai University of Agriculture and Engineering, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangzhou 510225 , China |
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| Abstract: | To explore the characteristics of energy metabolism in Macrobrachium rosenbergii and their relationship with locomotive abilities under salinity stress, an experiment with four salinity levels 0 (control), 4, 8, and 12] was conducted. Oxygen consumption rates, key energy metabolism enzyme activity levels in gills and muscles, and swimming and tail-flipping abilities were determined after 48 hours of salinity stress. The results showed that there were no significant differences in oxygen consumption rates as salinity increased from 0 to 4; however, the values were significantly higher in the 8 and 12 salinity groups when compared with those in the control group (0 salinity). There were no significant differences in the activity levels of hexokinase (HK), phosphofructokinase (PFK), and lactate dehydrogenase (LDH) in the gill among different salinity level groups. However, the activity levels of pyruvate dehydrogenase (PDH) and cytochrome c oxidase (COX) in the gill were significantly elevated as salinity increased from 0 to 8. Moreover, the activity levels of PDH and COX in the gill were positively correlated with oxygen consumption rates. The activity levels of HK, PFK, and LDH in pleopod and abdominal muscles did not change significantly when salinity increased from 0 to 8, but PDH and COX activity levels in pleopod and abdominal muscles significantly decreased under the same condition. Significant decreases in the activity levels of HK, PFK, LDH, PDH, and COX were observed as salinity increased from 0 to 12, either in pleopod or abdominal muscles. With regard to locomotion, swimming ability significantly decreased when salinity increased from 0 to 8; meanwhile, the same decrease for tail-flipping ability was observed at a salinity of 12. Swimming ability was positively correlated with PDH and COX activity levels of the pleopod muscles, whereas tail-flipping ability was positively correlated with the activity levels of HK, PFK, and LDH in abdominal muscles. Our findings indicate that as a response to salinity stress, M. rosenbergii increases its reliance on aerobic metabolism in the gill, as evidenced by the increase in oxygen intake. However, these physiological regulations reduce the enzyme activity levels associated with energy metabolism in muscles, resulting in observed decrease in swimming and tail-flipping abilities of M. rosenbergii. |
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