| 栉孔扇贝神经节一氧化氮合酶的组织化学和免疫组化定位 |
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| 引用本文： | 孙虎山,王宜艳,王晓安,孙修勤,李光友.栉孔扇贝神经节一氧化氮合酶的组织化学和免疫组化定位[J].中国水产科学,2005,12(1):20-25. |
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| 作者姓名： | 孙虎山 王宜艳 王晓安 孙修勤 李光友 |
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| 作者单位： | 1. 烟台师范学院,生命科学学院,山东,烟台,264025
2. 国家海洋局,第一海洋研究所,山东,青岛,266061 |
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| 基金项目： | 国家"973"计划资助项目(G1999012005). |
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| 摘 要： | 采用组织化学和免疫组化技术对栉孔扇贝(Chlamys farreri)神经节内的一氧化氮合酶(NOS)进行定位研究。组织化学显示，存在NOS的部位如下：脑神经节内纵行的神经纤维和表层的少量小细胞；足神经节表层的大量小细胞，中央大量水平分布的神经纤维；脏神经节中部大量水平分布的神经纤维，前叶内大量小细胞和神经纤维，后叶内少量小细胞和许多环行神经纤维，侧叶内大量似放射状分布的神经纤维；脑足和脑脏神经索内的神经纤维。免疫组化定位表明，神经型一氧化氮合酶(nNOS)和诱导型一氧化氮合酶(iNOS)在整个神经系统内均呈阴性；足神经节和脏神经节内有少量神经细胞呈内皮型一氧化氮合酶(eNOS)强阳性；各神经节和神经索内的部分小细胞和神经纤维呈eNOS弱阳性。栉孔扇贝进化上为较低等的贝类，NOS阳性神经细胞应主要分布于外周器官组织内。神经系统内大量的NOS可在其神经传导和免疫调节等方面发挥重要的作用。
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| 关 键 词： | 栉孔扇贝 神经节 一氧化氮合酶 组织化学 免疫组化 |
| 文章编号： | 1005-8737-(2005)01-0020-06 |
| 修稿时间： | 2004年1月13日 |
Histochemical and immunohistochemical localization of nitric oxide synthase in nervous ganglia of scallop Chlamys farreri |
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| SUN Hu-shan.Histochemical and immunohistochemical localization of nitric oxide synthase in nervous ganglia of scallop Chlamys farreri[J].Journal of Fishery Sciences of China,2005,12(1):20-25. |
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| Authors: | SUN Hu-shan |
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| Institution: | SUN Hu-shan~ |
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| Abstract: | The nitric oxide synthase (EC1.14.13.39; NOS) in the nervous ganglia of scallop Chlamys farreri were studied with histochemical and immunohistochemical localization method.The results indicate that the longitudinal nerve fibers in cerebral ganglia and a few of small cells in the surface layer of cerebral ganglia present NOS positive reaction.Abundant NOS positive small cells are in the surface layer of pedal ganglia,and abundant transverse positive nerve fibers in the center of pedal ganglia.A large number of transverse positive nerve fibers are in the center of visceral ganglia; abundant positive small cells and nerve fibers are in two anterior lobes; a few of positive small cells and many encircled positive nerve fibers are in the posterior lobe; a large number of radiate positive nerve fibers are in the lateral lobes.Positive nerve fibers are in pedal nerve cords and visceral nerve cords.There are no neuronal nitric oxide synthase (nNOS) or inducible nitric oxide synthase (iNOS) positive cells or fibers in the nervous system.A few of endothelial nitric oxide synthase (eNOS) strongly positive nerve cells are identified in pedal ganglia and visceral ganglia.Weakly positive cells and nerve fibers of eNOS can be seen in all of the ganglia and nerve cords.Chlamys farreri is a primary mollusc in evolution that a large number of NOS positive nerve cells must be located in the tissues of peripheral organs.The NOS in nervous system of scallop may play an important role in its nerve conduction and immune regulation. |
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| Keywords: | Chlamys farreri nervous ganglia nitric oxide synthase histochemistry immunohistochemistry |
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