| 盐碱对大鳞鳃血清渗透压、离子含量及鳃丝Na+/K+-ATP酶活力的影响 |
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| 引用本文： | 耿龙武,徐伟,李池陶,蔺玉华,刘晓勇,孙慧武,张永旺.盐碱对大鳞鳃血清渗透压、离子含量及鳃丝Na+/K+-ATP酶活力的影响[J].中国水产科学,2011,18(2). |
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| 作者姓名： | 耿龙武 徐伟 李池陶 蔺玉华 刘晓勇 孙慧武 张永旺 |
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| 作者单位： | 1. 中国水产科学研究院,黑龙江水产研究所,黑龙江,哈尔滨,150070
2. 中国水产科学研究院鲟鱼繁育技术工程中心,北京,100039 |
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| 基金项目： | 农业部"948"引进项目，黑龙江水产研究所基本科研业务费专项，黑龙江水产研究所基本科研业务费专项 |
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| 摘 要： | 通过室内毒理实验,研究了盐度、碱度以及盐碱交互作用对大鳞9E(Barbus capito)血清渗透压、血清离子(Na~+,K~+,CI~-、尿素氮)浓度和鳃丝Na~+/K~+ -ATP酶活力的影响.单因子实验中,随着含盐水平(8 g/L,10g/L、12g/L,14 g/L)的增大,大鳞鳃血清渗透压和血清离子(Na~+,K~+,Cl~-)浓度均显著升高(P<0.05),鳃丝Na~+/K~+-ATP酶活力先升高后降低,受体内外渗透压差的影响最显著(P<0.05).碱度的增大(19.05 mmol/L,30.20 mmol/L,47.86mmol/L,75.86 mmol/L)能引起血清K~+和尿素氮浓度显著升高(P<0.05),但对血清渗透压和鳃丝ATP酶活力无显著影响.在双因子实验中,盐碱交互升高会引起渗透压和血清离子(Na~+,CI~-、尿素氮)显著升高(P<0.05).方差分析结果表明,盐度、碱度及交互作用均对渗透压有显著影响,影响作用最大的是盐度,其次是碱度,交互作用最小.鳃丝Na~+/K~+-ATP酶活性先升高后降低,最高值出现在盐度10 g/L、碱度30.20 mmol/L的正交试验组.从实验结果可得出,大鳞鳃在盐度12g/L以下的水体中能生存,在盐碱共存的环境下,能耐受的上限为盐度10 g/L、碱度30.20mmol.本研究旨在掌握大鳞鳃的生存盐碱度范围,以期为该物种的增养殖生产提供基础依据.
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| 关 键 词： | 大鳞鲃 渗透压 血清离子浓度 Na+/K+-ATP酶 |
Influence of salinity and alkalinity on serum ion concentrations,serum osmosis and gill Na+/K+-ATPase activity of Barbus capito |
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| GENG Longwu,XU Wei,LI Chitao,LIN Yuhua,LIU Xiaoyong,SUN Huiwu,ZHANG Yongwang.Influence of salinity and alkalinity on serum ion concentrations,serum osmosis and gill Na+/K+-ATPase activity of Barbus capito[J].Journal of Fishery Sciences of China,2011,18(2). |
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| Authors: | GENG Longwu XU Wei LI Chitao LIN Yuhua LIU Xiaoyong SUN Huiwu ZHANG Yongwang |
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| Institution: | GENG Longwu 1,XU Wei 1,LI Chitao 1,LIN Yuhua 1,LIU Xiaoyong 2,SUN Huiwu 2,ZHANG Yongwang 2 1.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China,2.Technological and Engineering Center of Sturgeon's Reproduction,Beijing 100039 |
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| Abstract: | In this paper,the serum osmosis,serum ions concentration and Na+/K-ATPase activity in branchial epithelium of Barbus capito were investigated in a ambient of salinity,alkalinity and orthogonal experiment.The results showed that osmolarity in serum and serum ions concentration(Na+,K+,Cl-)were elevated significantly with the rising salinities(8,10,12,14g/L).The gill Na+/K+-ATPase activity increased in the beginning and decreased subsequently,Na+/K+-ATPase activity was positively related to the difference betw... |
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| Keywords: | barbus capito osmolarity serum ions Na /K -ATPase |
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