| 利用DNA条形码COI基因分析我国重要贝类系统进化关系 |
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| 引用本文： | 赵庆,吴彪,刘志鸿,刘寒苗,孙秀俊,周丽青,张高伟,杨爱国.利用DNA条形码COI基因分析我国重要贝类系统进化关系[J].中国水产科学,2018,25(4):847-857. |
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| 作者姓名： | 赵庆 吴彪 刘志鸿 刘寒苗 孙秀俊 周丽青 张高伟 杨爱国 |
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| 作者单位： | 农业农村部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所;水产科学国家级实验教学示范中心上海海洋大学;海洋渔业科学与食物产出过程功能实验室青岛海洋科学与技术国家实验室 |
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| 基金项目： | 科技部科技基础性工作专项（2013FY110700）；国家水产种质资源共享服务平台（2017DKA30470）. |
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| 摘 要： | DNA条形码旨在通过PCR技术获得一段DNA序列,在物种水平上对现存生物类群和未知生物材料进行识别和鉴定。线粒体细胞色素C氧化酶I(COI)基因是常用DNA条形码基因之一,为研究COI基因作为DNA条形码在贝类系统进化中的评估效果,本文利用PCR技术扩增获得了60个贝类物种的353条COI基因序列,通过聚类法构建了neighbor-joining(NJ)进化树,同时还对7个物种不同地理群体的遗传进化情况进行了分析。结果表明,选用的COI基因引物在大多数贝类中通用性较强,除在珍珠贝目中的扩增效率只有10%以外,在整个研究中扩增效率达到82.7%;60个物种中除太平洋潜泥蛤(Panopea abrupta)、沼蛤(Limnoperna fortunei)和魁蚶(Scapharca broughtoni)等8个物种在进化树中的进化地位与传统系统分类具有一定差别外,其他物种的聚类关系与传统分类基本一致;对7个物种、共26个地理群体的聚类分析发现,COI基因基本能对同一物种的不同地理群体进行聚类,只有极个别群体或群体中的某个个体存在聚类混乱现象。综上所述,COI基因在一定程度上适用于贝类物种鉴别和系统发育研究,丰富了COI基因在物种鉴别应用中的科学数据。
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| 关 键 词： | DNA条形码 线粒体COI 贝类 系统进化 |
| 修稿时间： | 2018/8/20 0:00:00 |
Analysis of evolutionary relationships of important shellfish in China based on the DNA barcoding COI gene |
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| ZHAO Qing,WU Biao,LIU Zhihong,LIU Hanmiao,SUN Xiujun,ZHOU Liqing,ZHANG Gaowei,YANG Aiguo.Analysis of evolutionary relationships of important shellfish in China based on the DNA barcoding COI gene[J].Journal of Fishery Sciences of China,2018,25(4):847-857. |
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| Authors: | ZHAO Qing WU Biao LIU Zhihong LIU Hanmiao SUN Xiujun ZHOU Liqing ZHANG Gaowei YANG Aiguo |
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| Institution: | 1. Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;2. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Techuology, Qingdao 266273, China;3. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | DNA barcodes can be used to identify existing biological groups and unknown biomaterials through shorter DNA sequences using polymerase chain reaction (PCR). The mitochondrial cytochrome coxidase I (COI) gene is one of the commonly used DNA barcoding genes. In the present study, to analyze the effectiveness of the COI gene for DNA barcoding in mollusk evolution, 353 sequences of 60 species belonging to 13 orders and 33 families were obtained by PCR amplification for clustering analysis. A neighbor-joining (NJ) phylogenetic tree was constructed by Mega, and the genetic evolution of seven species from different populations was also analyzed. The results showed that the universal primers of the COI gene were highly versatile for most shellfish. Although the amplification efficiency of primers in Pterioida was only 10%, it reached 82.7% for the individuals. Except for the evolutionary status of eight species, such as Scapharca broughtonii, Limnoperna fortunei, and Panopea abrupta, the clustering relationships of other species were similar to results obtained using traditional methods. Based on the clustering analysis of 7 species from 26 geographical groups, it was found that the COI gene generally clustered the different geographical groups of the same species first, and some individuals in the population or group had clustering disorder. In conclusion, the COI gene could be applied to the identification and phylogenetic studies of shellfish species to a certain extent, which would enhance the role of the COI gene in practical applications of species identification, and provide reference data for the construction of shellfish DNA barcoding databases. |
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| Keywords: | DNA barcoding COI gene sequence Mollusca evolution |
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