| 三种养殖石首鱼类的DNA条形码鉴定及有效种名确认 |
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| 引用本文： | 李昂,柳淑芳,安长廷,万瑞景,庄志猛.三种养殖石首鱼类的DNA条形码鉴定及有效种名确认[J].中国水产科学,2022,29(11):1541-1550. |
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| 作者姓名： | 李昂 柳淑芳 安长廷 万瑞景 庄志猛 |
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| 作者单位： | 中国水产科学研究院黄海水产研究所, 农业农村部海洋渔业可持续发展重点实验室, 山东 青岛 266071 ；青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室, 山东 青岛 266071 |
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| 基金项目： | 国家现代农业产业技术体系资助项目(CARS-47)； 中国水产科学研究院基本科研业务费资助项目(2021JC01)； 国家海洋水产种质资源库项目. |
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| 摘 要： | 针对部分养殖石首鱼种质资源存在命名混乱、物种鉴定不准确的问题, 本研究在形态学观察与测量的基础上, 利用 DNA 条形码技术对 3 种养殖石首鱼类进行了物种鉴定。测序获取待测样品 DNA 条形码序列 15 条, 在 GenBank 中对序列进行相似性比对分析, 同时在中国重要渔业生物 DNA 条形码信息平台验证了比对结果的准确性; 结合已报道的 18 条石首鱼类 DNA 条形码序列对全部样品进行分析, 运用 Kimura 2-paramater (K2P)模型构建其系统进化关系, 进一步确定待测样品的种类及分类地位。研究结果将 3 种养殖石首鱼分别定种为黄唇鱼Bahaba taipingensis (Herre, 1932)]、元鼎黄姑鱼(Nibea chui Trewavas, 1971)和双棘原黄姑鱼Protonibea diacanthus (Lacepède, 1802)], 厘清了 3 个物种的有效种名及分类特征, 证实了石首鱼外部形态、鳔、耳石的典型特征可作为其物种鉴定的重要证据, 对 DNA 条形码物种鉴定具有辅助作用, 表明 DNA 条形码技术可解决石首鱼类幼鱼由于形态特征尚不明显等问题造成的定种困难。研究结果为国家一级保护野生动物黄唇鱼的繁殖驯养报备和登记提供了科学证据, 也为石首鱼类种质资源鉴定、评价及其开发和保护提供了技术支撑。
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| 关 键 词： | 石首鱼类 DNA 条形码 物种鉴定 有效种名 |
Species identification and taxonomic classification of three cultured sciaenid species based on DNA barcoding |
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| LI Ang,LIU Shufang,AN Changting,WAN Ruijing,ZHUANG Zhimeng.Species identification and taxonomic classification of three cultured sciaenid species based on DNA barcoding[J].Journal of Fishery Sciences of China,2022,29(11):1541-1550. |
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| Authors: | LI Ang LIU Shufang AN Changting WAN Ruijing ZHUANG Zhimeng |
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| Abstract: | The family Sciaenidae (sciaenid), commonly referred to fishes such as croakers and drums, is a large taxonomic group in the order Perciformes. The sciaenids are commonly found in shallow coastal waters of tropical to temperate seas, and a few species inhabit freshwater. There are 27 sciaenid species belonging to 14 genera in the coastal waters of China and most of them are commercially important, both in capture and aquaculture fisheries. Due to the high levels of similarity between related species and the paucity of observable morphological features among juvenile fish, species delineation and identification are difficult in the sciaenid species. Thus, the traditional morphological identification method cannot meet the needs of species identification. Meanwhile, there are several synonyms and invalid species names in the published taxa of sciaenid aquaculture species. The above situations bring confusion to the taxonomic study and aquaculture production of sciaenid fish. In recent years, DNA barcoding has been suggested as a useful molecular technique to complement traditional taxonomic expertise and provides an important tool for species identification and classification. In this study, we used DNA barcoding for effective identification of juveniles from three cultured sciaenid species (including 15 samples from the same aquaculture system) and confirmed their valid species names. The preliminary identification of the three species was based on the analyses of the external morphology and morphology of the swim bladder and otolith. Meanwhile, we examined the DNA barcode fragment of 610 bp of the mitochondrial cytochrome c oxidase subunit I (CO I) gene. BLAST searches were performed to identify similar sequences in GenBank. Homologous sequences from closely related species were also included in the comparative analysis and species identification. Phylogenetic relationships among nine species of the family Sciaenidae were reconstructed based on neighborjoining (NJ) based Kimura-2 parameter (K2P) net genetic distance. It was shown that the results of morphological and DNA barcoding analysis were consistent, indicating that the samples from three species could be identified as Bahaba taipingensis (Herre, 1932), Nibea chui Trewavas, 1971 and Protonibea diacanthus (Lacepède, 1802) respectively. We conclude that DNA barcoding can be used to identify the species of juvenile individuals in the family Sciaenidae and the morphology analysis could play an important supporting role. All the three species in this study have been classified as endangered or vulnerable species in the China Species Red List, and the effective species identification and the clarification of valid species names provide scientific basis for the conservation and utilization of their germplasm resources. |
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