| 中国近海与日本近海白姑鱼线粒体细胞色素b基因全序列比较分析 |
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| 引用本文： | 李昂,高天翔,孙典荣.中国近海与日本近海白姑鱼线粒体细胞色素b基因全序列比较分析[J].中国水产科学,2010,17(6):1166-1172. |
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| 作者姓名： | 李昂 高天翔 孙典荣 |
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| 作者单位： | 1. 中国海洋大学,海水养殖教育部重点实验室,山东,青岛,266003
2. 中国水产科学研究院,南海水产研究所,广东,广州,510300 |
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| 基金项目： | 国家自然科学基金项目 |
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| 摘 要： | 对中国近海和日本近海共19尾白姑鱼(Pennahia argentata)线粒体细胞色素b(Cytochrome b,Cytb)基因全序列进行扩增与测定,白姑鱼个体的Cytb基因总长度均为1141bp。序列分析结果显示:Cytb基因全序列中共检测到40处核苷酸替代,全部为同义替换,且主要来自密码子第三位点。19尾白姑鱼个体共定义了14种单倍型。核苷酸组成分析表明:白姑鱼Cytb全序列的鸟嘌呤(G)含量较低,在第三密码子位点上表现得尤为明显。基于邻接法、最大简约法、最大似然法和贝叶斯法构建的系统树结果基本一致,中国近海和日本近海的白姑鱼明显分为2个单系群,两组群间的净遗传距离为0.019,基于Cytb2%/百万年分子钟计算,其分化时间约为95万年,中、日近海白姑鱼分化事件发生于晚更新世(Late Pleistocene)。本研究旨在探讨中、日近海白姑鱼组群的分类地位的遗传学依据,为白姑鱼渔业资源的管理与保护提供基础参考。
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| 关 键 词： | 白姑鱼 细胞色素b基因 遗传距离 分歧时间 中国近海 日本近海 |
| 修稿时间： | 2010/11/15 0:00:00 |
Comparative analysis of white croaker (Pennahia argentata) based on complete cytochrome b gene sequence in mitochondrial DNA |
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| LI Ang,GAO Tianxiang,SUN Dianrong.Comparative analysis of white croaker (Pennahia argentata) based on complete cytochrome b gene sequence in mitochondrial DNA[J].Journal of Fishery Sciences of China,2010,17(6):1166-1172. |
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| Authors: | LI Ang GAO Tianxiang SUN Dianrong |
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| Institution: | LI Ang1,GAO Tianxiang1,SUN Dianrong2(1.The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,China,2.South China Sea Fisheries Research Institute,Chinese Academy of Fishery Science,Guangzhou 510300,China) |
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| Abstract: | The mitochondrial cytochrome b(Cyt b) gene of Pennahia argentata from different locations was amplified by PCR and the sequence with total length of 1411 bp was sequenced and analyzed.According to the analysis,two distinct clades were detected,one in China's offshore waters and another in the Japan's offshore waters.Forty nucleotide substitutions were checked in the total sequences analyzed,and most of them were synonymous transitions at the third codon positions.Nucleotide composition analysis indicated a ... |
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| Keywords: | Pennahia argentata cytochrome b gene genetic distances divergence time Japan's offshore China's off shore |
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