| 副溶血弧菌对凡纳滨对虾肝胰腺抗氧化酶活性和基因表达的影响 |
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| 引用本文： | 赵伟,王雷,刘梅,蒋克勇,齐灿灿,杨广,王宝杰.副溶血弧菌对凡纳滨对虾肝胰腺抗氧化酶活性和基因表达的影响[J].中国水产科学,2017,24(6):1261-1270. |
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| 作者姓名： | 赵伟 王雷 刘梅 蒋克勇 齐灿灿 杨广 王宝杰 |
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| 作者单位： | 1. 中国科学院 海洋研究所, 中国科学院实验海洋生物学重点实验室, 山东 青岛 266071;天津农学院 水产学院, 天津市水产生态及养殖重点实验室, 天津 300384;2. 中国科学院 海洋研究所, 中国科学院实验海洋生物学重点实验室, 山东 青岛 266071;青岛海洋科学与技术国家实验室海洋生物学与生物技术功能实验室, 山东 青岛 266071;3. 天津农学院 水产学院, 天津市水产生态及养殖重点实验室, 天津 300384 |
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| 基金项目： | 国家科技支撑计划项目(2015BAD17B03)，山东省科技重大专项(2015ZDZX05002) |
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| 摘 要： | 为了研究致病性副溶血弧菌(Vibrio parahaemolyticus)对凡纳滨对虾(Litopanaeus vannamei)肝胰腺抗氧化酶活性及相关基因表达的影响,本研究选取始体重(2.20±0.24)g的健康凡纳滨对虾初270尾,将其随机分为2组,分别以0 CFU/m L和5×107 CFU/m L剂量的致病性副溶血弧菌对凡纳滨对虾进行浸浴攻毒实验。在实验第6小时、12小时、24小时和36小时取肝胰腺,进行抗氧化酶活性及免疫相关基因表达测定。结果显示,感染副溶血弧菌后,实验组对虾肝胰腺中超氧化物歧化酶(SOD)活性显著高于对照组(P0.05),而过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)和谷胱甘肽-S转移酶(GST)活性随感染时间的增加呈现先上升后下降的趋势,CAT活性于第12小时达到最大值,而GSH-PX和GST分别在第6小时和第24小时达到最大值,此结果表明副溶血弧菌感染对凡纳滨对虾肝胰腺抗氧化酶活性有显著影响,对其机体免疫防御系统有明显的破坏作用。Rab蛋白基因(Rab)和干扰素-维甲酸联合应用诱导细胞凋亡相关基因(Grim-19)表达水平显著高于对照组(P0.05),抗菌肽crustin和酚氧化酶原基因(Pro PO)的表达水平呈现先升高后降低趋势,分别在第12小时和第24小时达到最大值,分别为11.4倍和28.2倍,而GST基因表达水平在第12~36小时显著低于对照组(P0.05)。m TOR信号通路中真核细胞始动因子4E结合蛋白(4EBP)基因的表达水产显著高于对照组(P0.05),真核细胞翻译启动因子1(e IF4E1A)和真核细胞翻译启动因子2(e IF4E2)基因的表达水平呈现先升高后降低的趋势,分别在第12小时和第6小时达到最大值,分别为2.6倍和1.6倍,核糖体S6蛋白激酶基因(P70s6k)的表达水平显著低于对照组(P0.05)。上述结果表明,凡纳滨对虾感染致病性副溶血弧菌后,肝胰腺抗氧化酶活性及免疫相关基因的表达均受到显著影响。
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| 关 键 词： | 凡纳滨对虾 副溶血弧菌 肝胰腺 抗氧化酶 基因表达 |
| 修稿时间： | 2017/11/25 0:00:00 |
The effects of Vibrio parahaemolyticus on hepatopancreas antioxidant enzyme activity and gene expression of Litopenaeus vannamei |
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| ZHAO Wei,WANG Lei,LIU Mei,JIANG Keyong,QI Cancan,YANG Guang,WANG Baojie.The effects of Vibrio parahaemolyticus on hepatopancreas antioxidant enzyme activity and gene expression of Litopenaeus vannamei[J].Journal of Fishery Sciences of China,2017,24(6):1261-1270. |
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| Authors: | ZHAO Wei WANG Lei LIU Mei JIANG Keyong QI Cancan YANG Guang WANG Baojie |
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| Institution: | 1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2. Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;3. College of Fishery Science, Tianjin Agriculture University;Tianjin Key Laboratory of Aquaculture, Tianjin 300384, China |
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| Abstract: | In order to determine the impact of pathogenic Vibrio parahaemolyticus (Vp) on the hepatopancreas of Litopenaeus vannamei, shrimps with weights of (2.2±0.24) g were challenged with Vp at a concentration of 5×107 CFU/mL for 36 h. The hepatopancreas tissues from the experimental and control groups were collected at 6 h, 12 h, 24 h, and 36 h after the start of the experiment to evaluate antioxidant enzyme activities and immune and mTOR signal pathway-related gene expression levels. The results showed that after challenge with Vp, SOD activity was significantly higher than that of the control group (P<0.05), while CAT, GSH-PX, and GST activities all increased at first and subsequently decreased, reaching the peak value at 12 h, 6 h and 24 h, respectively (P<0.05). The rela-tive expression levels of Rab and Grim-19 were significantly higher than in the control group (P<0.05), crustin and ProPO were up-regulated at first and then decreased after reaching their peaks at 12 h and 24 h, respectively, while the level of the GST gene was significantly lower than that of the control group during the time 12-36 h (P<0.05). These results showed that functions of the immune system were affected by Vp. Several important genes in mTOR signal pathway were also been quantified by qRT-PCR. The relative expression levels of 4EBP gene were significantly higher than those of the control group (P<0.05). eIF4E1A and eIF4E2 were both up-regulated at first and then decreased after reaching peak values at 12 h and 6 h, respectively (P<0.05). The level of P70s6k was significantly lower than that of the control group after challenge (P<0.05). The results indicated that Vp challenge had a noticeable impact on antioxidant enzyme activities and immune and mTOR signal pathway-related gene expression of Litopanaeus vannamei, with significant destructive effect on the shrimp's immune system. |
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| Keywords: | Litopanaeus vannamei Vibrio parahaemolyticus hepatopancreas antioxidase gene expression |
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