| 邻苯二甲酸二丁酯对红鳍笛鲷幼鱼的胁迫效应 |
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| 引用本文： | 秦洁芳,陈海刚,蔡文贵,杨涛,贾晓平.邻苯二甲酸二丁酯对红鳍笛鲷幼鱼的胁迫效应[J].中国水产科学,2011,18(5):1125-1131. |
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| 作者姓名： | 秦洁芳 陈海刚 蔡文贵 杨涛 贾晓平 |
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| 作者单位： | 中国水产研究院南海水产研究所广东省渔业生态环境重点实验室农业部南海渔业资源环境重点野外科学观测实验站;上海海洋大学海洋科学学院; |
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| 基金项目： | 科技部科研院所社会公益研究专项(2005DIB3J021); 中央级公益性科研院所基本科研业务费专项资金项目(2009YD01,2010YD04); 广东省科技计划项目(2009B030600001); 中国水产科学研究院淡水生态与健康养殖重点开放实验室开放课题(2010FEA03006) |
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| 摘 要： | 为了探讨邻苯二甲酸二丁酯(DBP)对红鳍笛鲷(Lutjanus erythropterus)幼鱼的胁迫效应,于DBP暴露后0、6、12、24、48和96 h时检测红鳍笛鲷鳃、肝和脑组织中超氧化物歧化酶(SOD)、丙二醛(MDA)和乙酰胆碱酯酶(AChE)活性。结果表明,DBP对红鳍笛鲷的24 h、48 h和96 h LC50分别为7.10 mg/L、6.98 mg/L和6.66 mg/L,安全质量浓度为2.04 mg/L。随着浓度增加,鳃组织中的SOD酶活性表现为先升高后下降,具有明显的时间效应;肝组织中的SOD酶活性在0.5 mg/L和2.0 mg/L浓度组交替表现为升高和降低,与对照组比较有显著性差异(P<0.05);而0.125 mg/L浓度组肝SOD没有明显变化。鳃组织中MDA含量在DBP暴露6 h后显著性增加(P<0.05),之后随时间延长而下降;DBP暴露6 h和12 h后,0.5 mg/L和2.0 mg/L浓度组肝组织中的MDA含量都显著升高(P<0.05),之后随时间延长MDA含量下降并趋于稳定。与对照组比较,DBP暴露48 h后,红鳍笛鲷脑组织中AChE酶活性显著升高(P<0.01),96 ...
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| 关 键 词： | 邻苯二甲酸二丁酯 LC50 超氧化物歧化酶 丙二醛 乙酰胆碱脂酶 |
| 修稿时间： | 2011/10/8 0:00:00 |
Effects of di-n-butyl phthalate (DBP) on activity of enzyms in different tissues of crimson snapper (Lutjanus erythropterus) |
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| QIN Jiefang,CHEN Haigang,CAI Wengui,YANG Tao,JIA Xiaoping.Effects of di-n-butyl phthalate (DBP) on activity of enzyms in different tissues of crimson snapper (Lutjanus erythropterus)[J].Journal of Fishery Sciences of China,2011,18(5):1125-1131. |
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| Authors: | QIN Jiefang CHEN Haigang CAI Wengui YANG Tao JIA Xiaoping |
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| Institution: | QIN Jiefang1,2,CHEN Haigang1,CAI Wengui1,YANG Tao1,JIA Xiaoping1 1.South China Sea Fisheries Research Institute,Chinese Aacademy of Fishery Science,Key Laboratory of Fishery Ecology Environment,Guangdong Province of China,Key Field Scientific Experimental Station of South China Fishery Resource and Environment,Ministry of Agriculture,Guangzhou 510300,China,2.College of Marine Science,Shanghai Ocean University,Shanghai 201306 |
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| Abstract: | Di-n-butyl phthalate(DBP),a member of the phthalate acid ester(PAE) family,is thought to have an endocrine disrupting effect on vertebrates and humans and has been identified as priority controlled hazardous substance in the United States and China.In order to evaluate the effect of DPB,we examined liver and gill levels of superoxide dismutase(SOD) and malondialdehyde(MDA),and brain levels of acetylcholinesterase(AChE) in the crimson snapper(Lutjanus erythropterus).Groups of fish were exposed to 0,0.125,0.5... |
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| Keywords: | di-n-butyl phthalate(DBP) LC50 superoxide dismutase malondialdehyde acetylcholinesterase |
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