| 苏丹鱼染色体核型及系统进化分析 |
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| 引用本文： | 曲照球,麻艳群,唐瞻杨,黄凯,肖俊,陈丽婷,黄婷,罗永巨,郭忠宝,黎明,陆专灵,高雪梅.苏丹鱼染色体核型及系统进化分析[J].中国水产科学,2023,30(7):821-830. |
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| 作者姓名： | 曲照球 麻艳群 唐瞻杨 黄凯 肖俊 陈丽婷 黄婷 罗永巨 郭忠宝 黎明 陆专灵 高雪梅 |
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| 作者单位： | 广西壮族自治区水产科学研究院, 广西 南宁 530021 ；广西大学动物科学技术学院, 广西 南宁 530004 |
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| 基金项目： | 国家自然科学基金项目(32260917)； 广西壮族自治区农业科技自筹经费项目(Z2022216) |
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| 摘 要： | 鱼类染色体核型和系统进化分析在鱼类种质资源保护和利用等方面发挥着重要作用,为丰富苏丹鱼(Leptobarbushoevenii)种质资源研究内容,本研究以苏丹鱼为研究对象,采用染色体冷滴片制备法分析了染色体核型特征,运用线粒体16S rRNA基因序列比对分析了其系统进化关系,探讨了鲃亚科鱼类种属间亲缘关系。染色体核型分析结果显示:苏丹鱼二倍体的染色体数为2n=50,其中包含中部着丝粒染色体(m)8条、亚中部着丝粒染色体(sm) 14条、亚端部着丝粒染色体(st) 2条和端部着丝粒染色体(t) 1条,核型公式为2n=16m+28sm+4st+2t,染色体臂数(NF)为94。14种鲃亚科鱼类的线粒体16SrRNA基因比对分析结果表明:鲃亚科鱼类的种间遗传距离为0.016～0.134,平均遗传距离为0.074;苏丹鱼与粗须白甲鱼(Onychostomabarbata)的种间遗传距离最大(0.134),与泰国短吻鱼(Sikukiastejnegeri)的种间遗传距离最小(0.090);系统进化关系结果显示:苏丹鱼位于进化树的基部,与四须鲃属亲缘关系较近。本研究结果可为苏丹鱼种质资源鉴定、品种改...
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| 关 键 词： | 苏丹鱼 染色体 核型分析 16S rRNA 系统发育 |
| 收稿时间： | 2023/5/4 0:00:00 |
| 修稿时间： | 2023/6/16 0:00:00 |
Analysis of chromosome karyotype and phylogenetic evolution on Leptobarbus hoevenii |
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| QU Zhaoqiu,MA Yanqun,TANG Zhanyang,HUANG Kai,XIAO Jun,CHEN Liting,HUANG Ting,LUO Yongju,GUO Zhongbao,LI Ming,LU Zhuanling,GAO Xuemei.Analysis of chromosome karyotype and phylogenetic evolution on Leptobarbus hoevenii[J].Journal of Fishery Sciences of China,2023,30(7):821-830. |
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| Authors: | QU Zhaoqiu MA Yanqun TANG Zhanyang HUANG Kai XIAO Jun CHEN Liting HUANG Ting LUO Yongju GUO Zhongbao LI Ming LU Zhuanling GAO Xuemei |
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| Abstract: | Leptobarbus hoevenii is an economically significant fish found in Malossia and worldwide that has been introduced to China owing to its high economic value and good aquaculture prospects. However, the existing culture population has been unselected and degraded in germplasm, generating a need for genetic breeding- related studies on L. hoevenii. Since karyotyping and phylogenetic analysis play an important role in the conservation and utilization of fish germplasm resources, the karyotype of L. hoevenii was prepared by the chromosome cold-drop method. In order to study the evolutionary and interspecific genetic relationship among 14 species of the Barbinae subfamily, the mitochondrial 16S rRNA gene sequences were compared and used to construct phylogenetic trees. The karyotype results of L. hoevenii showed a diploid chromosome number of 2n=50, containing 8 metacentric (m) chromosomes, 14 submetacentric (sm) chromosomes, 2 subtelocentric (st) chromosomes, and 1 telocentric (t) chromosome. Furthermore, the number of chromosome arms (NF) was 94, revealing the karyotype formula to be 2n=16m+28sm+4st+2t, NF=94. Regarding the comparison of mitochondrial 16S rRNA genes of the 14 Barbidae species, the genetic distances ranged from 0.016 to 0.134, with an average of 0.074. Notably, the interspecific genetic distances between L. hoevenii and Onychostoma barbata was the largest at 0.134, while that between L. hoevenii and Sikukia stejnegeri was the smallest at 0.090. Finally, the resulting phylogenetic tree showed that these 14 species were clustered into two respective branches, indicating a closer relationship between L. hoevenii and Barbodes hexagonlepis. L. hoevenii was basal in the phylogenetic tree. These results provide a foundation for the evaluation, breeding, and phyletic evolution of fish germplasm resources. |
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