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| 河蟹精子膜蛋白的生化特性 | |
| 引用本文： | 王琦,康现江,程立均,穆淑梅,曹刚.河蟹精子膜蛋白的生化特性[J].中国水产科学,2010,17(1). |
| 作者姓名： | 王琦 康现江 程立均 穆淑梅 曹刚 |
| 作者单位： | 1. 河北大学,生命科学学院,河北,保定,071002;长治学院,生物科学与技术系,山西,长治,046011 2. 河北大学,生命科学学院,河北,保定,071002 3. 河北大学,生命科学学院,河北,保定,071002;华北制药集团新药研究开发有限责任公司,河北,石家庄,050015 4. 河北大学,生命科学学院,河北,保定,071002;保定市白洋淀亚行贷款项目管理办公室,河北,保定,071051 |
| 基金项目： | 国家自然科学基金资助项目，河北省自然科学基金资助项目 |
| 摘 要： | 利用含1% TritonX-100和0.1%SDS的提取缓冲液提取河蟹(Eriocheir sinensis)精子膜蛋白。对所得的精子膜蛋白溶液进行SDS-PAGE电泳分析。结果显示,河蟹的精子含有12种不同的膜蛋白组分。对这些精子膜蛋白组分的生化特性进行分析,结果表明,河蟹精子膜蛋白是1组呈酸性的低分子量糖蛋白,分子量为21.6～75.5kD,等电点在4.8左右。 |
| 关 键 词： | 河蟹 精子膜蛋白 生化特性 |
Biochemical characteristic of sperm membrane proteins in Eriocheir sinensis |
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| WANG Qi,KANG Xianjiang,CHENG Lijun,MU Shumei,CAO Gang.Biochemical characteristic of sperm membrane proteins in Eriocheir sinensis[J].Journal of Fishery Sciences of China,2010,17(1). | |
| Authors: | WANG Qi KANG Xianjiang CHENG Lijun MU Shumei CAO Gang |
| Institution: | WANG Qi 1,2,KANG Xianjiang 1,CHENG Lijun 1,3,MU Shumei 1,CAO Gang 1,4(1.College of Life Sciences,Hebei University,Baoding 071002,China,2.Department of Biology&Chemistry,Changzhi University,Changzhi 046011,3.North China Pharmacy Group New Medicine Research , Develop Co.Ltd.,Shijiazhuang 050015,4.Loan Project Management Office of Asian Development Bank of Baiyang Lake of Baoding,Baoding 071051,China) |
| Abstract: | Sperm membrane proteins of Eriocheir sinensis are extracted with extraction buffer containing 1% Triton X-100 and 0.1% SDS.Then the proteins were analyzed by SDS-PAGE.Results show the sperm membrane proteins of E.sinensis include 12 different kinds of proteins.Biochemical characteristics of these sperm membrane proteins are analyzed,and the results show that their molecular weights are between 21.6 kD and 75.5 kD,and the isoelectric points(PI) are about 4.8,which indicates the sperm membrane proteins of E.s... |
| Keywords: | Eriocheir sinensis sperm membrane protein biochemical characteristics |
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