| 显微注射生长激素基因导入中国对虾（Penaeus chinensis）受精卵的研究 |
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| 引用本文： | 刘萍,孔杰,王清印,李健,杨丛海,梁利群,孙孝文.显微注射生长激素基因导入中国对虾（Penaeus chinensis）受精卵的研究[J].中国水产科学,1996,3(4):35-38. |
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| 作者姓名： | 刘萍 孔杰 王清印 李健 杨丛海 梁利群 孙孝文 |
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| 作者单位： | [1]中国水产科学研究院黄海水产研究所 [2]中国水科科学研究孟黑龙江水产研究所 |
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| 摘 要： | 本实验采用显微微量注射方法，交羊生长激素基因导入中轻地虾受精卵，受精卵发育到蚤状幼体第三期后采样检测。ＰＣＲ检测结果表明：７个样品共９３尾幼体，有３个样品呈出阳性信号，基因转移比率至少在３％以上。同时斑点杂交结果也证明有两个明显阳性斑点。
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| 关 键 词： | 显微注射法 生长激素 基因 中国对虾 受精卵 |
| 修稿时间： | 2010/4/8 0:00:00 |
THE STUDIES ON THE FERTILIZED EGG OF PENAEUS CHINENSIS WITH MICROINJECTION OF THE GROWTH HORMORE GENE |
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| Liu Ping,Kong Jie,Wang Qingyin,Li Jian,Yang Conghai.THE STUDIES ON THE FERTILIZED EGG OF PENAEUS CHINENSIS WITH MICROINJECTION OF THE GROWTH HORMORE GENE[J].Journal of Fishery Sciences of China,1996,3(4):35-38. |
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| Authors: | Liu Ping Kong Jie Wang Qingyin Li Jian Yang Conghai |
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| Abstract: | Sheep growth hormone gene was introduced into fertilized eggs of shrimp (Penaeus chinensis) by microinjection. Samples of Z 3 were collected and tested by PCR. Test results showed that three of the groups (total larval number is 93) were positive. and therefore, it is believed that the gene transfer efficiency is more than 3%. The two positive samples were tested by dot blot and the results were just the same as the two of the three mentioned above. |
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| Keywords: | Microinjection Growth hormone gene Shrimp PCR Dot blot |
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