Effect of Acute Hypoxia in Trout (<Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis>) on Immature Erythrocyte Release and Production of Oxidative Radicals |
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| Authors: | Email author" target="_blank">A?ValenzuelaEmail author V?Silva E?Tarife?o A?Klempau |
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| Institution: | (1) Ph.D. Program in Biological Science, Fish Culture Laboratory, Oceanography Department, University of Concepción, Barrio Universitario s/n, Casilla 160-C, Concepción, Chile;(2) Faculty of Pharmacy, Clinical Biochemistry and Immunology Department, University of Concepción, Concepción, Chile;(3) Faculty of Natural Sciences and Oceanography, Zoology Department, University of Concepción, Concepción, Chile;(4) Faculty of Natural Sciences and Oceanography, Oceanography Department, University of Concepción, Concepción, Chile |
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| Abstract: | This paper evaluates the response of trout exposed to acute hypoxia between 8.7 mgO2/l (controls) and 1.4 mgO2/l. Temperature, pH, NH4, oxygen consumption rate and spleen-somatic index (SSI) were evaluated. Blood cell counts, oxidative
radicals (ORs) production and immature erythrocytes were assessed to evaluate their relationship with the degree of hypoxia.
Acute hypoxia induced a decrease in water pH (p < 0.05), oxygen consumption rate (60% less), SSI (55% less) and a significant
increase of water ammonium concentration (p < 0.05). Haematocrit increased significantly starting at 4.8 mgO2/l, as well as an increase in the total number of leukocytes (up to 50% in some fish). Starting at 2 mgO2/l, swelling of erythrocytes was induced and a decrease in the potential production of ORs, showing a negative correlation
to immature erythrocyte numbers. These results suggest that lower than normal oxygen concentrations may jeopardize ORs production
and hence non-specific defence mechanisms in trout. High percentages of immature erythrocytes can be used as a simple and
inexpensive indicator of both hypoxic events and impairment of ORs production in the absence of bleeding, haemolysis, anti-anaemia
treatments and/or increased temperature. |
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| Keywords: | erythrocytes haematology hypoxia Oncorhynchus mykiss oxidative radicals |
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