Effects of the aromatase inhibitor Fadrozole on plasma sex steroid secretion and oocyte maturation in female coho salmon (Oncorhynchus kisutch) during vitellogenesis |
| |
Authors: | LOB Afonso GK Iwama J Smith EM Donaldson |
| |
Institution: | (1) West Vancouver Laboratory, Fisheries and Oceans Canada, 4160 Marine Drive, West Vancouver, B.C, V7V 1N6, Canada;(2) Department of Animal Science and the Canadian Bacterial Diseases Network, 248-2357 Main Mall, Vancouver, B.C, V6T 1Z4, Canada |
| |
Abstract: | 17-estradiol, 17-20-dihydroxy-4-pregnen-3-one (17-20-P), and testosterone levels were measured in plasma samples obtained from vitellogenic coho salmon (Oncorhynchus kisutch) before and 32 days after injection of the aromatase inhibitor Fadrozole (AI). Plasma 17-estradiol levels decreased significantly 6 h after injection in all AI treated fish. The higher the dose the longer the maintenance of low plasma 17-estradiol levels. Inversely, plasma 17-20-P increased significantly 6 h after injection in all AI treated fish, and the higher the dose the longer the maintenance of high plasma 17-20-P levels. At 48 h after injection plasma testosterone levels were significantly higher in the AI treated groups. The oocyte maturation index showed that multiple injections with AI retarded oocyte development. Besides, oocyte diameter and GSI were lower in the same group, which presented high incidence of atresia of vitellogenic oocytes. The ovarian follicles and brain of the fish which received multiple injections secreted less 17-estradiol, in vitro. These findings suggest that aromatase inhibitors such as Fadrozole may have a potential as a tool to regulate sexual development in salmon. |
| |
Keywords: | aromatase atresia brain oocytes 17-estradiol" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-estradiol 17-20gif" alt="agr" align="BASELINE" BORDER="0">-20-dihydroxy-4-pregnen-3-one" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-dihydroxy-4-pregnen-3-one salmon testosterone |
本文献已被 SpringerLink 等数据库收录! |
|