Optimisation of sodium and potassium concentrations and pH in the artificial seminal plasma of common carp <Emphasis Type="Italic">Cyprinus carpio</Emphasis> L. |
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| Authors: | Beata Irena Cejko Ákos Horváth Timea Kollár Eszter Kása Jelena Luji? Zoran Marinovi? Béla Urbányi Rados?aw Kajetan Kowalski |
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| Institution: | 1.Department of Gamete and Embryo Biology,Institute of Animal Reproduction and Food Research, Polish Academy of Science,Olsztyn,Poland;2.Department of Aquaculture, Institute of Aquaculture and Environmental Safety,Szent István University,G?d?ll?,Hungary |
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| Abstract: | The effect of sodium and potassium concentrations as well as optimal pH on the motility of common carp Cyprinus carpio L. sperm during short-term storage in artificial seminal plasma (ASP) was investigated. Sperm was collected from individual males (n?=?5) and each sample diluted tenfold (1:9) in ASP (sperm:extender) containing 2 mM CaCl2, 1 mM Mg2SO4 and 20 mM Tris at pH 8.0 and supplemented by the following concentrations of sodium and potassium (mM/mM): 0/150, 20/130, 40/110, 75/75, 110/40, 130/20 and 150/0. The osmolality of all ASP variants was set at 310 mOsm kg?1. Sperm motility was measured using a CASA system during 72 h of storage. Immediately after dilution, sperm motility was high (90%) both in each variant and in the control group (fresh sperm). After 72-h storage, the highest sperm motility was noted in ASP containing 110 mM NaCl and 40 mM KCl. No differences were found in the motility of samples preserved within the pH range of 7.0–9.0. Our data suggest that for the short-term storage of common carp sperm, whereas the pH of the solution does not play a crucial role, a specific potassium concentration of around 40 mM is required. |
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