Effects of metomidate anaesthesia or transfer to pur sea water on plasma parameters in ammonia-exposed Atlantic salmon (Salmo salar L) in sea water |
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| Authors: | M B Knoph |
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| Institution: | (1) Department of Morphology, Genetics and Aquatic Biology, Norwegian College of Veterinary Medicine, Box 8146 Dep, N-0033 Oslo, Norway |
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| Abstract: | Atlantic salmon (Salmo salar L) postsmolts weighing 150 ± 53 g were exposed to 14–15 mg l–1 TA-N (total ammonia-N) in sea water in 1 m3 tanks for 24h. Blood samples were then taken A) immediately after the fish were netted from the exposure tanks and stunned by a blow to the head; B) 2–20 min after the fish were transferred to 15 l of an anaesthetic solution of metomidate in  ammonia-free sea water; or C) 2–20 min after the fish were transferred to 15 l of ammonia-free sea water. Plasma TA-N level was 18% lower in the anaesthetised fish compared to in the fish sampled directly from the exposure tanks (p  0.05), and accordingly 16% lower in the fish transferred to pure sea water although this difference was not significant (p = 0.07). Plasma glucose level was higher in the fish transferred to pure sea water than in the fish receiving the two other treatments (p 0.05), but plasma urea, osmolality, Na+, Cl–, Ca2+ or Mg2+ levels did not vary significantly between the different treatments. Plasma TA-N level increased with time in the fish in the metomidate solution (p 0.02). |
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| Keywords: | teleost Salmo salar metomidate anaesthesia blood sampling handling ammonia urea glucose osmolality ions |
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