| 应用环境DNA技术对邵伯湖浮游动物物种检测的初步研究 |
| |
| 引用本文： | 唐晟凯,钱胜峰,沈冬冬,张彤晴,刘燕山,许飞,王华,李大命.应用环境DNA技术对邵伯湖浮游动物物种检测的初步研究[J].水产养殖,2021(3):13-20. |
| |
| 作者姓名： | 唐晟凯 钱胜峰 沈冬冬 张彤晴 刘燕山 许飞 王华 李大命 |
| |
| 作者单位： | 江苏省淡水水产研究所;江苏省高宝邵伯湖渔业管理委员会办公室 |
| |
| 基金项目： | 江苏省水生生物资源重大专项暨首次水生野生动物资源普查(ZYHB16-3)。 |
| |
| 摘 要： | 为掌握邵伯湖浮游动物物种组成,利用环境DNA技术于2018年11月对其浮游动物群落进行了监测。在邵伯湖15个采样点同步进行了水样采集,抽滤后进行了DNA提取,利用线粒体DNA 12S r RNA引物进行PCR扩增,并进行了高通量测序。结果显示,从二代测序的1114482条DNA序列中,比对得到浮游动物OTUs数68个。利用宏条形码共检出浮游动物22种(隶属于14科18属),邵伯湖浮游动物功能群以轮虫滤食者RF为主,各样点浮游动物α和β多样性的各项指数较为均匀,表明邵伯湖各区域的浮游动物多样性水平较接近。环境DNA技术适用于浅水湖泊浮游动物群落监测,在现阶段的淡水浮游动物资源监测中,该技术宜与传统的监测方法结合使用。
|
| 关 键 词： | 邵伯湖 环境DNA 物种检测 浮游动物 |
Preliminary study on detection of zooplankton species in Shaobo Lake by environmental DNA technology |
| |
| Tang Shengkai,Qian Shengfeng,Shen Dongdong,Zhang Tongqing,Liu Yanshan,Xu Fei,Wang Hua,Li Daming.Preliminary study on detection of zooplankton species in Shaobo Lake by environmental DNA technology[J].Journal of Aquaculture,2021(3):13-20. |
| |
| Authors: | Tang Shengkai Qian Shengfeng Shen Dongdong Zhang Tongqing Liu Yanshan Xu Fei Wang Hua Li Daming |
| |
| Institution: | (Freshwater Fisheries Research Institute of Jiangsu Province,Nanjing 210017,China;Lake GaoBaoshaobohu Fishery Management Commission of Jiangsu Province,Yangzhou 225009,China) |
| |
| Abstract: | In order to understand the species composition of zooplankton in Shaobo lake, the zooplankton community was monitored by environmental DNA technology in November 2018. Water samples were collected at15 sampling sites in Shaobo lake, and DNA was extracted after filtration. PCR amplification was performed using mitochondrial DNA 12 S rRNA primers, and high-throughput sequencing was performed. The results showed that68 OTUs were obtained from 1114482 DNA sequences. Twenty two species of zooplankton(22 species, 18 genera,14 families) were detected by metabarcoding. Rotifer filter feeders were the main functional groups of zooplankton in Shaobo lake. The α and β diversity indexes of zooplankton in different sites were relatively uniform, which indicated that the diversity level of zooplankton in each region of Shaobo Lake was close. Environmental DNA technology is suitable for the monitoring of zooplankton community in shallow lakes. In the current monitoring of freshwater zooplankton resources, this technology should be combined with traditional monitoring methods. |
| |
| Keywords: | Shaobo Lake environmental DNA species detection zooplankton |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
