| 粉红螺旋聚孢霉NF-06固体发酵条件优化及对南方根结线虫的防治效果 |
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| 引用本文： | 张洁,郭雪萍,夏明聪,孙润红,武超,刘红彦,杨丽荣,张猛.粉红螺旋聚孢霉NF-06固体发酵条件优化及对南方根结线虫的防治效果[J].中国生物防治学报,2020,36(1):105-112. |
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| 作者姓名： | 张洁 郭雪萍 夏明聪 孙润红 武超 刘红彦 杨丽荣 张猛 |
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| 作者单位： | 1. 河南省农业科学院植物保护研究所/河南省生物农药工程研究中心/河南省作物保护国际联合实验室, 郑州 450002;2. 河南农业大学, 郑州 450002 |
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| 基金项目： | 河南省科技攻关专项（182102110272）；河南省农业科学院科研发展专项资金（2019CY02）；河南省农业科学院自主创新基金项目（20192C33） |
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| 摘 要： | 南方根结线虫是一种严重危害我国蔬菜安全生产的土传病害，生产上亟需一种有效的防控方法。本研究从根结线虫卵囊中分离到一株生防真菌NF-06，通过形态学和分子生物学方法将其鉴定为粉红螺旋聚孢霉Clonostachys rosea。通过离体活性测定的方法测定NF-06发酵滤液对根结线虫2龄幼虫的致死作用，发现24、48和72 h后2龄幼虫的相对死亡率分别达63.6%，75.5%和87.0%。以产孢量为指标，优化菌株NF-06固体发酵条件，确定最佳基础培养基为麦麸和麦秸秆混合物，其比例为4：1（wt/wt），在基础培养基中添加10%玉米粉、2%蚕豆粉和0.05% KH2PO4可获得最大产孢量；最优培养条件为初始pH值7.0，初始含水量40%，接种量5%，产孢量达8.9×1010 CFU/g。室内盆栽防治效果测定显示，菌株NF-06固体发酵物能够有效降低土壤中的线虫数量和番茄根结指数，其中5 g/株穴施处理的线虫减退率和防治效果分别达67.9%和69.6%。本研究为生物杀线虫剂的开发和利用提供理论依据。
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| 关 键 词： | 根结线虫 粉红粘帚霉 生物防治 发酵条件优化 |
| 收稿时间： | 2019-05-09 |
Optimization of Solid State Fermentation Conditions of Clonostachys rosea NF-06 and Its Control Efficiency on Meloidogyne incognita |
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| ZHANG Jie,GUO Xueping,XIA Mingcong,SUN Runhong,WU Chao,LIU Hongyan,YANG Lirong,ZHANG Meng.Optimization of Solid State Fermentation Conditions of Clonostachys rosea NF-06 and Its Control Efficiency on Meloidogyne incognita[J].Chinese Journal of Biological Control,2020,36(1):105-112. |
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| Authors: | ZHANG Jie GUO Xueping XIA Mingcong SUN Runhong WU Chao LIU Hongyan YANG Lirong ZHANG Meng |
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| Institution: | 1. Henan Biopesticide Engineering Research Center/Henan International Joint Laboratory of Crop Protection/Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;2. Henan Agricultural University, Zhengzhou 450002, China |
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| Abstract: | Root-knot nematode Meloidogyne incognita is an important soil-borne disease that threatens the safety of vegetables. Effective control measures are in urgent need. In this study, a biocontrol fungal strain NF-06 was isolated from the egg masses of M. incognita, identified as Clonostachys rosea by morphological and rDNA-ITS sequence analysis. An initial assay of NF-06 culture filtrates on second-stage juveniles was conducted, which showed relative mortalities of 63.6%, 75.5% and 87.0% at 24 h, 48 h and 72 h post treatment, respectively. The solid state fermentation conditions of the strain NF-06 were optimized using the conidium number as an index. The results showed that the optimal basal medium was the mixture of wheat bran and wheat straw, with a mass ratio of 4:1 (wt/wt). When corn flour (10%), broad bean (2%) and KH2PO4(0.05%) were supplemented into the basal medium, the maximum conidial production was achieved. The viable conidial number reacheed 8.9×1010 CFU/g at the optimal culture conditions of initial pH 7.0, initial water content 40%, and inoculum dose 5%. The NF-06 solid culture significantly reduced the population densities of juveniles in soil and root-knot index in tomato in a greenhouse test. When hole applied at 5 g per plant, the NF-06 solid culture afforded reduction rates of juveniles and control efficiency of 67.9% and 69.6%, respectively. This study provides a basis for the research and development of bio-nematicide. |
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| Keywords: | root-knot nematode Clonostachys rosea biological control fermentation condition optimizition |
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