| 转基因检测标准物质研制关键环节质量控制研究 |
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| 引用本文： | 高芳瑞,王颢潜,李瑞环,刘娜,王永,武玉花,李亮,赵新,梁晋刚.转基因检测标准物质研制关键环节质量控制研究[J].中国生物防治学报,2022,38(5):1125-1134. |
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| 作者姓名： | 高芳瑞 王颢潜 李瑞环 刘娜 王永 武玉花 李亮 赵新 梁晋刚 |
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| 作者单位： | 1. 天津市农业科学院种质资源与生物技术研究所, 天津 300381;2. 农业农村部科技发展中心, 北京 100176;3. 中国农业科学院油料作物研究所, 武汉 430062;4. 中国农业科学院农业质量标准与检测技术研究所, 北京 100098 |
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| 基金项目： | 转基因生物重大专项(2016ZX08012003-007);农业科研杰出人才培养计划(2021年) |
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| 摘 要： | 在转基因产品检测方法开发、身份鉴定和产品成分定性、定量检测过程中，标准物质是不可缺少的物质基础。本研究对标标准物质申报要求，着重对比了基体标准物质、基因组DNA标准物质、质粒标准物质三种类型转基因检测标准物质的研制过程，提出关键环节的质量控制要求，为促进转基因检测标准物质研制过程标准化、提升研制报告编制质量、提高申报通过率提供技术支撑。
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| 关 键 词： | 转基因检测 基体标准物质 质粒DNA标准物质 基因组DNA分子标准物质 关键环节 |
| 收稿时间： | 2022-07-15 |
Research on Key Link and Critical Control Point of GMO Testing Reference Material |
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| GAO Fangrui,WANG Haoqian,LI Ruihuan,LIU Na,WANG Yong,WU Yuhua,LI Liang,ZHAO Xin,LIANG Jingang.Research on Key Link and Critical Control Point of GMO Testing Reference Material[J].Chinese Journal of Biological Control,2022,38(5):1125-1134. |
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| Authors: | GAO Fangrui WANG Haoqian LI Ruihuan LIU Na WANG Yong WU Yuhua LI Liang ZHAO Xin LIANG Jingang |
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| Institution: | 1. Institute of Germplasm Resources and Biotechnology, Tianjin Academy of Agriculture Science, Tianjin 300381, China;2. Development Center of Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing 100176, China;3. Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China;4. Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100098, China |
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| Abstract: | In the process of GMO testing development,identification and product composition qualitative and quantitative testing,reference material is the indispensable material basis.This study focuses on the application requirements of standard substances,and compares the development process of three types of transgenic standard substances,namely,matrix standard substances,genomic DNA standard substances and plasmid standard substances.Put forward the quality control requirement of key link,it provides technical support for promoting the standardization of the development process of standard materials for transgenic testing,improving the quality of development report preparation and improving the application rate. |
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| Keywords: | GMO test primary reference material plasmid DNA material genome DNA material key tache |
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