Screening for Differential Gene Expression in Atropa belladonna Leafy Gall Induced Following Rhodococcus fascians Infection |
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| Authors: | El Hassan Nouar Danny Vereecke Koen Goethals Mondher Jaziri Marie Baucher |
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| Institution: | (1) Laboratory of Plant Biotechnology, Université Libre de Bruxelles, Chaussée de Wavre 1850, B-1160 Brussels, Belgium;(2) Plant Systems Biology, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium |
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| Abstract: | To better characterise at the molecular level the nature of plant responses to infection by Rhodococcus fascians PCR-based differential display patterns of Atropa belladonna leafy gall (LG) and non-infected plant tissues were compared. Six differentially expressed genes were identified and their altered expression was confirmed by RT-PCR. Three of them corresponded to up-regulated genes which encode proteins involved in plant defence. The three remaining cDNA fragments which correspond to down-regulated genes in LG, encoded proteins with similarity to a multicystatin, a miraculin and a methallothionein-like protein, respectively. Upon elimination of the bacteria from infected plant tissue, the expression of up-regulated genes was maintained, whereas expression of down-regulated genes resumed suggesting a potential role of these up-regulated genes in plant growth and development. |
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| Keywords: | differential display phytopathogenic bacteria PR proteins |
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