Characterization of Erwinia amylovora Strains from Croatia |
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| Authors: | Edyta Halupecki Carlo Bazzi Susanne Jock Klaus Geider Damir ?ermi? Bogdan Cvjetkovi? |
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| Institution: | (1) Department of Plant Pathology, Faculty of Agriculture, Svetošimunska 25, 10000 Zagreb, Croatia;(2) Department of Agroenvironmental Sciences and Technologies, University of Bologna, Viale Fanin 42, 40127 Bologna, Italy;(3) Max Planck Institute Ladenburg, Schwabenheimer Str. 101, D-69221 Dossenheim, Germany;(4) Federal Biological Research Centre for Agriculture and Forestry (BBA), Schwabenheimer Str. 101, D-69221 Dossenheim, Germany;(5) Department of Molecular Biology, Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, Croatia |
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| Abstract: | Erwinia amylovora is the causative agent of fire blight, a destructive disease of rosaceous plants. The European population can be divided
into several subtypes according to differences in restriction fragment length polymorphism of the XbaI genomic DNA digest analysed with pulsed-field gel electrophoresis. This technique was also used to determine the genetic
relatedness of six Croatian isolates to the E. amylovora types found in the countries surrounding Croatia. The isolates belong to the Pt2 pattern type that is characteristic of the
East Mediterranean basin. All tested isolates gave essentially the same total cell protein pattern in SDS-polyacrylamide gel
electrophoresis. The number of short-sequence DNA repeats in plasmid pEA29 of six isolates was determined by PCR assays and
ranged from four to seven. The isolates examined showed high pathogenicity in immature pear fruits. Differences were also
revealed in microbiological assays such as amylovoran synthesis, levan formation, siderophore production and colour on coliform
medium. |
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| Keywords: | fire blight pathogenicity PFGE SSR |
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