| 黄连根腐病发生与根际土壤、根茎内生、叶内生细菌群落结构变化关系 |
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| 引用本文： | 唐 涛,袁 斌,王帆帆,郭 杰,郭晓亮,段媛媛,游景茂.黄连根腐病发生与根际土壤、根茎内生、叶内生细菌群落结构变化关系[J].植物保护,2022,48(1):73-81. |
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| 作者姓名： | 唐 涛 袁 斌 王帆帆 郭 杰 郭晓亮 段媛媛 游景茂 |
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| 作者单位： | 1. 湖北省农业科学院中药材研究所, 恩施 445000; 2. 湖北省农业科学院植保土肥研究所, 武汉 430064 |
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| 基金项目： | 农业农村部华中作物有害生物综合防治重点实验室/农作物重大病虫草害防控湖北省重点实验室开放基金(2020ZTSJJ6);湖北省农业科技创新中心项目(2020 620 003 001);恩施州研发计划(D20190015);湖北省农业科学院中药材研究所青年基金(2020ZYCJJ03) |
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| 摘 要： | 为了探讨细菌群落结构与黄连根腐病发生的关系,本文分析了两个不同地点健康和染病黄连的根茎、叶内生细菌和根际土壤细菌的组成和差异。结果显示,黄连根茎、叶片和根际土壤样品中根际土壤细菌群落多样性最丰富,根茎内生菌次之。在所有样品中,主要的优势菌门为变形菌门Proteobacteria、放线菌门Actinobacteria、拟杆菌门Bacteroidetes和绿弯菌门Chloroflexi。相较于健康黄连组样品,染病黄连根际土壤、根茎和叶片样品中多个菌属细菌相对丰度发生显著改变,如Vibrionimonas,乳杆菌属Lactobacillus和鞘氨醇单胞菌属Sphingomonas等,表明可能与黄连根腐病的发生有一定关联。此外,RDA分析表明,伯克氏菌属Burkholderia、全噬菌属Holophaga和雷尔氏菌属Ralstonia与黄连根际土壤有机质、有效氮、pH有显著相关性。本研究分析了两个不同地点的健康和染病黄连根际、叶片内生细菌和根际土壤细菌群落结构差异,为综合分析黄连根腐病的成因和机制提供了参考。
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| 关 键 词： | 黄连 根腐病 细菌群落结构 高通量测序 多样性 |
| 收稿时间： | 2020/12/6 0:00:00 |
| 修稿时间： | 2021/1/4 0:00:00 |
Relationships between the occurrence of root rot and the change in the community structure of rhizosphere soil bacteria, rhizome and leaf endophytic bacteria of Coptis chinensis |
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| TANG Tao,YUAN Bin,WANG Fanfan,GUO Jie,GUO Xiaoliang,DUAN Yuanyuan,YOU Jingmao.Relationships between the occurrence of root rot and the change in the community structure of rhizosphere soil bacteria, rhizome and leaf endophytic bacteria of Coptis chinensis[J].Plant Protection,2022,48(1):73-81. |
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| Authors: | TANG Tao YUAN Bin WANG Fanfan GUO Jie GUO Xiaoliang DUAN Yuanyuan YOU Jingmao |
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| Institution: | 1. Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi 445000, China; 2. Institute of Plant Protection, Soil and Fertilizer, Hubei Academy of Agricultural Sciences, Wuhan 430064, China |
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| Abstract: | To explore the relationships between the bacterial community structure and the occurrence of Coptis chinensis root rot, the composition and diversity of endophytic bacteria in rhizomes, leaves and rhizosphere soils bacteria of healthy and infected Coptis chinensis in two different locations were analyzed. The results showed that the rhizosphere soil bacterial communities had the greatest diversity, followed by endophytic bacteria in rhizomes. Overall, the dominant phyla were Proteobacteria, Actinobacteria, Bacteroidetes and Chloroflexi. Compared with bacteria from the healthy C.chinensis samples, the relative abundance of bacteria of multiple genera detected in rhizosphere soil, rhizome and leaf samples from infected C.chinensis changed significantly, for example Vibrionimonas, Lactobacillus, Sphingomonas, etc., suggested that these bacteria might be related to the occurrence of C.chinensis root rot. In addition, the RDA analysis showed that Burkholderia, Holophaga, Ralstonia, etc. were significantly correlated with organic matter, available nitrogen and pH value of rhizosphere soil. This study analyzed the composition and diversity of endophytic bacteria in rhizomes, leaves, and bacteria in rhizosphere soil of C.chinensis from two different locations, and provided a theoretical basis for the comprehensive analysis of the causes and mechanisms of C.chinensis root rot. |
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| Keywords: | Coptis chinensis root rot bacterial community structure high throughput sequencing diversity |
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