| 铜绿假单胞菌ZJ1999对水稻纹枯病的防治及其在水稻上的定殖 |
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| 引用本文： | 任小平,谢关林,王笑.铜绿假单胞菌ZJ1999对水稻纹枯病的防治及其在水稻上的定殖[J].中国生物防治,2006,22(1):54-57. |
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| 作者姓名： | 任小平 谢关林 王笑 |
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| 作者单位： | 1. 广东省植物检疫站,广州,510500
2. 浙江大学生物技术研究所,杭州,310029 |
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| 基金项目： | 中国科学院资助项目;亚洲开发银行(ADB)资助项目 |
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| 摘 要： | 利用拮抗菌铜绿假单胞菌ZJ1999产生的粗提液进行防治水稻纹枯病研究的结果表明，抑制病菌侵染的活力随着粗提液浓度提高和处理时间的延长而增强。该菌株的定殖时间与最初引进时的浓度密切相关，其最低应用浓度为10^8cfu／ml，在接种纹枯病病菌后第一天的喷施防效最佳。拮抗菌ZJ1999在发病的秧苗、成株期植株茎杆和叶片上，14d后的菌量分别维持在10^2-10^3和10^3～10^4cfu／g。
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| 关 键 词： | 水稻纹枯病 铜绿假单胞菌 定殖 生物防治 |
| 文章编号： | 1005-9261(2006)01-0054-04 |
| 收稿时间： | 2005-01-18 |
| 修稿时间： | 2005年1月18日 |
Application and Colonization of Pseudomonas aeruginosa ZJ1999 for Biocontrol of Rhizoctonia solani, Pathogen of Rice Sheath Blight |
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| REN Xiao-ping,XIE Guan-lin,WANG Xiao.Application and Colonization of Pseudomonas aeruginosa ZJ1999 for Biocontrol of Rhizoctonia solani, Pathogen of Rice Sheath Blight[J].Chinese Journal of Biological Control,2006,22(1):54-57. |
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| Authors: | REN Xiao-ping XIE Guan-lin WANG Xiao |
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| Institution: | Plant Quarantine Station of Guangdong Province, Guangzhou 510500, China |
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| Abstract: | The crude extracts from the antagonistic bacterium Pseudomonas aeruginosa ZJ1999 were tested for effectiveness against Rhizoctonia solani, pathogen of rice sheath blight. Results showed that the inhibition ability against R. solani varied with concentration of tile extracts and the treatment time for mycelium of R. solani. The colonization duration of the antagonistic bacterium on rice plants was closely related to the initial concentration. The population of P. aeruginosa ZJ1999 could retain at 10^2 - 10^3 cfu/g and 10^3 - 10^4 cfu/g 14 d .after spraying on seedling and adult plants. Minimum concentration of the extract was 10^8 cfu/ml, and the optimum spraying time was at the first day after inoculation of R. solan for best control of rice sheath blight. |
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| Keywords: | Rhizoctonia solani Pseudomonas aeruginosa ZJ1999 colonization biocontrol |
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