| 基于DPPH法对紫茎泽兰提取物抗氧化活性的研究 |
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| 引用本文： | 胡楚娇,王崇云,和兆荣,朱薇,黄罗冬.基于DPPH法对紫茎泽兰提取物抗氧化活性的研究[J].杂草科学,2013(4):9-12. |
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| 作者姓名： | 胡楚娇 王崇云 和兆荣 朱薇 黄罗冬 |
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| 作者单位： | 云南大学生命科学学院,云南昆明650091 |
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| 基金项目： | 国家自然科学基金（编号：31160080、31260078）. |
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| 摘 要： | 以维生素c为对照，采用DPPH法研究紫茎泽兰叶和茎醇提物的抗氧化活性，并计算它们的Ic20。结果表明：1．6mg／mL紫茎泽兰叶和茎的醇提物有很好的清除能力，清除率均达到80％以上，相当于0．10mg／mL维生素C的清除率；紫茎泽兰叶和茎的Ic50为0．3．0．7mg／mL，维生素c为0．04mg／mL。说明紫茎泽兰叶的清除能力强于茎，紫茎泽兰清除自由基的能力为维生素c的1／10。总之，紫茎泽兰具有较强的抗氧化活性。
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| 关 键 词： | 紫茎泽兰 提取物 抗氧化活性 维生素c |
Study on Antioxidant Activity of Extracts from Ageratina adenophora based on DPPH Method |
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| HU Chu-jiao,WANG Chong-yun,HE Zhao-rong,ZHU Wei,HUANG Luo-dong.Study on Antioxidant Activity of Extracts from Ageratina adenophora based on DPPH Method[J].Weed Science,2013(4):9-12. |
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| Authors: | HU Chu-jiao WANG Chong-yun HE Zhao-rong ZHU Wei HUANG Luo-dong |
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| Institution: | ( School of Life Science, Yunnan University, Kunming65009 , China) |
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| Abstract: | With vitamin C as a reference, the antioxidant activity of ethanol extracts from the leaves and stems of Ageratina adenophora （ Spreng. ） R. M. King ＆ H. Rob. were studied by the DPPH method and their ICso values were calculated. Extracts of both leaves and stems were good scavengers. At 1.6 mg/mL, extracts exhibited the best scavenging activity at above 80%. Their capacities equaled 0.10 mg/mL vitamin C. While the ICso values of leaves and stems were 0.3 and 0.7 mg/mL, that of vitamin C was 0.04 mg/mL. This indicated that the scavenging capacities of leaf extracts were better than stem extracts,and that the scavenginglcapacities of A. adenophora extracts was about 1/10 of vitamin C. Thus A. adenophora had stronger antioxidant activity than vitamin C. |
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| Keywords: | Ageratina adenopltora 3preng extracts antioxidant vitamin C |
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