Methods for visualizing hyaline fungi for the detection of antifungal compounds on thin-layer chromatograms |
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| Authors: | S WOODWARD R B PEARCE |
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| Institution: | Department of Forestry, University of Oxford, Commonwealth Forestry Institute, South Parks Road, Oxford OX1 3RB, UK |
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| Abstract: | Two methods facilitating the use of hyaline fungi for the detection of antifungal compounds on thin-layer chromatograms are described. Fungal growth was visualized under ultraviolet light either by the metabolic release of fluorescein from the esterase substrate fluorescein diacetate, or by the binding of a fluorescent brightener (Calcofluor White M2R New, or Tinopal-CBS) to the mycelium on the TLC plate.
These methods were used to demonstrate compounds inhibitory to the wood-decay fungus Heterobasidion annosum in bark extracts of Sitka spruce ( Picea sitchensis ). |
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