Comparison of mobile and stationary spore-sampling techniques for estimating virulence frequencies in aerial barley powdery mildew populations |
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| Authors: | M S HOVMØLLER L MUNK H ØSTERGÅRD |
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| Institution: | Danish Institute of Plant and Soil Science, Department of Plant Pathology and Pest Management, Lottenborgvej 2, DK-2800 Lyngby;Plant Pathology Section, Department of Plant Biology. Thorvaldsensvej 40, The Royal Veterinary and Agricultural University, DK-1871 Frederiksberg C;Plant Genetics Section, Environmental Science and Technology Department. Riso National Laboratory. Frederiksborgvej 399, DK-4000 Roskilde, Denmark |
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| Abstract: | Gene frequencies in samples of aerial populations of barley powdery mildew ( Erysiphe graminis f.sp. hordei ), which were collected in adjacent barley areas and in successive periods of time, were compared using mobile and stationary sampling techniques. Stationary samples were collected from trap plants in three periods within 1 week at a distance of more than 1000 m from the nearest barley field. At four dates within the same 8-day period, other samples were collected by a mobile spore trap along four sampling routes of a total distance of 130 km around the stationary stand of exposure. The samples were characterized by virulence genotypes defined according to infection types on 12 near-isogenic barley lines, and frequencies of single virulence genes were subsequently calculated. The three samples collected at the stationary site differed significantly with respect to allele frequencies at three loci. The main wind direction was different in the three sampling periods, implying different powdery mildew sources. For the mobile exposure, the differences between routes were not significant for any locus. However, the routes differed most for the loci under direct selection by host resistances genes, indicating a different distribution of source varieties along routes. There was no difference between allele frequencies at different dates, indicating that the proportions of spores from different source varieties were similar at these dates. In conclusion, samples collected by the stationary technique will mainly reflect the source varieties present in the local area, whereas samples collected by the mobile spore trap will mainly reflect sources close to the sampling route. Therefore, sampling sites as well as sampling routes should be defined such that source varieties are representative for the overall varietal distribution in the survey region considered. |
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