Monilinia species identified on peach and nectarine in Croatia,with the first record of Monilinia fructicola |
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| Authors: | Dario Ivi? Tina Fazini? Jennifer Cole Adrijana Novak |
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| Institution: | 1. Croatian Centre for Agriculture, Food and Rural Affairs, Institute for Plant Protection, , 10000 Zagreb, Croatia;2. The Food and Environment Research Agency, , York, YO41 1LZ UK |
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| Abstract: | During 2012, an official survey was conducted for Monilinia species present on peach and nectarine in Croatia. In total, 169 Monilinia spp. isolates were collected from 24 commercial orchards and identified according to morphology in culture and PCR. Eighty of the isolates were identified as Monilinia laxa, 70 as M. fructigena and 19 as M. fructicola. M. fructicola was found only at one location in the Mediterranean part of the country, and this is the first record of this quarantine fungus in Croatia. PCR diagnostic tests using M. fructicola‐specific primer pair MO368‐5/MO368‐10R repeatedly gave false negatives for some isolates. PCR tests using primer pair ITS1‐Mfc1/ITS4‐Mfc1 amplified M. fructicola‐specific product in all isolates and was therefore shown to be more suitable for diagnostic purposes. |
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