| 水稻纹枯病发病过程PR1和PBZ1的表达动态 |
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| 引用本文： | 赵长江,鲁国东,杜晓昱,刘丽,林艳,时玉君,王宗华.水稻纹枯病发病过程PR1和PBZ1的表达动态[J].植物病理学报,2006,36(4):317-321. |
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| 作者姓名： | 赵长江 鲁国东 杜晓昱 刘丽 林艳 时玉君 王宗华 |
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| 作者单位： | 1 福建农林大学生物农药与化学生物学教育部重点实验室, 福州 350002;2 福建农林大学植物保护学院, 福州 350002;3 福建农林大学生命科学学院, 福州 350002 |
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| 摘 要： | 采用从福建省稻田分离纯化的纹枯病菌(Rhizoctonia solani)菌株FJ-15接种籼稻9311,分析了水稻在纹枯病菌侵染致病过程中,编码病程相关蛋白的基因表达动态,并观察了症状的变化。Northern blot分析表明:PR1在接种12 h后开始表达,在之后的4个时间段其表达量逐步增强;而PBZ1也在12 h开始表达,在48 h表达量激剧增强几乎与72 h表达量相当。组织和症状观察表明,接种12 h后叶鞘表面菌丝纵横分枝,接种36 h后出现零星病斑,接种48 h后表现典型的受害症状,接种72 h后病斑继续扩大,并可蔓延到非接种叶鞘。结果表明,PR1和PBZ1的表达与水稻和纹枯病菌亲和互作的过程存在对应关系。
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| 关 键 词： | 水稻纹枯病 纹枯病菌 病程相关蛋白基因 侵染过程 |
| 文章编号： | 0412-0914(2006)04-0317-05 |
| 修稿时间： | 2005年1月10日 |
PR1 and PBZ1 expression through the infection process of rice sheath blight |
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| ZHAO Chang-jiang,LU Guo-dong,DU Xiao-yu,LIU Li,LIN Yan,SHI Yu-jun,WANG Zong-hua.PR1 and PBZ1 expression through the infection process of rice sheath blight[J].Acta Phytopathologica Sinica,2006,36(4):317-321. |
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| Authors: | ZHAO Chang-jiang LU Guo-dong DU Xiao-yu LIU Li LIN Yan SHI Yu-jun WANG Zong-hua |
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| Institution: | 1 The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2 College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China;3 The School of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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| Abstract: | To investigate the pathogenesis-related gene(PR) expression through the infection process of rice sheath blight,rice cultivar 9311 was inoculated with the fungal pathogen Rhizoctonia solani isolated and purified from the breeding field of Fujian Agriculture and Forestry University in Fuzhou,China.Rice samples were collected at 0,4,12,24,36,48,72 h post-inoculation for total RNA extraction and observation of di-(sease) development.Two PR gene fragments,PR1 and PBZ1,were used as probes for Northern blot analysis.The results showed that expression of both PR1 and PBZ1 was induced at 12 h post-inoculation,however PR1 was increased gradually from 12 h to 72 h post-inoculation,while PBZ1 drastically increased at 48 h post-ino-(culation) to almost the maximum of 72 h post-inoculation.Analysis of infection process showed that mycelium grew randomly on the surface of tissue at 12 h post-inoculation,few lesions started to come out at 36 h post-(inoculation),and typical lesions were found in all plants at 48 h post-inoculation.Integrating the results of mole-(cular) analysis and infection progress observation,we concluded that expression of PR1 and PBZ1 may be rela-(ted) with disease development of rice sheath blight,which may suggest that SA signal is involved in the defense pathway. |
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| Keywords: | rice sheath blight Rhizoctonia solani PR gene infection process |
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