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| 乙烯信号参与调控水稻纹枯病抗性的研究 | |
| 引用本文： | 薛芗,冯志明,曹文磊,王雨,陈宗祥,马玉银,张亚芳,潘学彪,左示敏.乙烯信号参与调控水稻纹枯病抗性的研究[J].植物病理学报,2020,50(4):462-470. |
| 作者姓名： | 薛芗 冯志明 曹文磊 王雨 陈宗祥 马玉银 张亚芳 潘学彪 左示敏 |
| 作者单位： | 扬州市职业大学,扬州 225000; 江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室/江苏省作物基因组学和分子育种重点实验室,扬州大学农学院,扬州 225009; 江苏省粮食作物现代产业技术协同创新中心,扬州大学,扬州 225009 |
| 基金项目： | 国家自然科学基金(31672013);江苏省高等学校自然科学研究项目(19KJD210001);霍英东青年教师基金项目(151026);江苏省自然科学基金面上项目(BK20171293);2017年度扬州市职业大学校级科研课题(2017ZR26);扬州市职业大学2018年度校级重点科研项目(yzpc2018014) |
| 摘 要： | 乙烯(ET)信号在植物抵抗各种逆境反应中具有重要作用,但目前关于乙烯信号在调控水稻纹枯病抗性中的作用仍缺乏系统研究。本研究以感纹枯病水稻品种Lemont和抗纹枯病水稻品种YSBR1为材料,分析了纹枯病菌接种后乙烯合成关键基因及信号传导途径中标志基因的表达水平,结果显示,纹枯病菌侵染可显著诱导相关基因的表达、激活乙烯信号。进一步采用乙烯合成抑制剂和信号激活剂处理水稻并进行接种鉴定,结果显示,两种化学剂分别可抑制或激活乙烯信号;无论是Lemont还是YSBR1,激活其乙烯信号均可显著增强抗病性,而抑制该信号均显著降低抗病性。对3个乙烯受体突变体(ethylene response2, etr2; ethylene response3, etr3; ethylene response sensor2, ers2)进行温室接种鉴定,发现突变体的病斑长度均极显著高于野生型对照。以上研究表明乙烯信号在水稻对纹枯病菌的防卫反应或基础抗性中具有十分重要的作用,结果将为进一步解析“水稻-纹枯病菌”间的互作机制、制定合适的病害综合防控策略提供理论基础。 |
| 关 键 词： | 水稻 纹枯病 抗性 乙烯信号 |
| 收稿时间： | 2019-10-22 |
A study on ethylene signaling involving in regulating resistance to rice sheath blight |
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| XUE Xiang,FENG Zhi-ming,CAO Wen-lei,WANG Yu,CHEN Zong-xiang,MA Yu-yin,ZHANG Ya-fang,PAN Xue-biao,ZUO Shi-min.A study on ethylene signaling involving in regulating resistance to rice sheath blight[J].Acta Phytopathologica Sinica,2020,50(4):462-470. | |
| Authors: | XUE Xiang FENG Zhi-ming CAO Wen-lei WANG Yu CHEN Zong-xiang MA Yu-yin ZHANG Ya-fang PAN Xue-biao ZUO Shi-min |
| Institution: | Yangzhou Polytechnic College, Yangzhou 225000, China; Jiangsu Key Laboratory of Crop Genetics and Physiology/ Key Laboratory of Plant Functional Genomics of the Ministry of Education/ Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Agricultural College of Yangzhou University, Yangzhou 225009, China; Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China |
| Abstract: | Ethylene (ET) signaling plays important roles in plant defense responses to various environmental stresses, but its role in regulation of rice sheath blight resistance remains to be studied systematically. In this study, a susceptible rice cultivar Lemont and a resistant rice cultivar YSBR1 were used to analyze the expression levels of marker genes in ethylene biosynthesis and signaling pathway after inoculation with R. solani. The results showed that the infection of R. solani significantly induced the expression levels of related genes and activated ethylene signaling pathway. Two chemicals were employed to activate and suppress ET signaling, respectively, on both cultivars. After inoculation with R. solani on both cultivars, we found that activation of ET signaling significantly enhance rice resistance to the pathogen, while suppress the ET signaling apparently reduced rice resistance. Further, we inoculated three ET receptor mutants, etr2, etr3, and ers2, with R. solani at the booting stage in greenhouse. As expect, we found that lesion lengths of the three mutants were all significantly longer than that of the wild type. Taken together, these data demonstrated that ET signaling played an important role in rice defense response or basic resistance to sheath blight. These results provide a theoretical basis for further elucidating the mechanism between "rice - R. solani” and establishing new strategies to prevent and control this disease. |
| Keywords: | rice sheath blight resistance ethylene signaling |
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