| miR535-3p调控水稻纹枯病抗性的功能研究 |
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| 引用本文： | 张朝阳,冯涛,左示敏,欧阳寿强.miR535-3p调控水稻纹枯病抗性的功能研究[J].植物病理学报,2022,52(5):791-800. |
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| 作者姓名： | 张朝阳 冯涛 左示敏 欧阳寿强 |
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| 作者单位： | 扬州大学园艺与植物保护学院,扬州大学农学院,扬州 225009 |
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| 基金项目： | 国家重点研发计划(2016YFD0100601) |
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| 摘 要： | 纹枯病是水稻生产中最重要的病害之一,其病原物立枯丝核菌(Rhizoctonia solani)是一种典型的土传真菌。前期我们通过立枯丝核菌侵染水稻后转录组测序,发现多个水稻内源miRNA响应R. solaniAG1-IA的侵染,其中Osa-miR535-3p的表达在R. solani侵染后被抑制。为明确Osa-miR535-3p在水稻应答R. solani侵染中的功能,本研究分别构建了Osa-miR535-3p在感病品种Xudao3背景下的敲除和过表达材料。发现与Xudao3相比,Osa-miR535-3p敲除系的纹枯病发病程度显著加重,而其过表达系的发病程度明显减轻。 Osa-miR535-3p敲除系的二级枝梗数目增多、穗长增加、分蘖数减少、千粒重增加,而其过表达系的株高降低、二级枝梗数目减少、穗长变短、分蘖数增多、千粒重下降,表明Osa-miR535-3p也参与调控水稻的农艺性状。通过RNA-seq通路分析,发现Osa-miR535-3p主要是通过影响植物激素信号通路如乙烯(Ethylene,ET)、吲哚乙酸(3-Indoleacetic acid,IAA)等信号相关基因的表达,调控水稻对纹枯病的抗性。本研究结果将为进一步解析水稻抗纹枯病分子机制提供理论参考,同时将为抗纹枯病水稻品种培育提供新思路。
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| 关 键 词： | 水稻 纹枯病 microRNA 抗病基因 Osa-miR535-3p |
| 收稿时间： | 2021-07-05 |
The function of Osa-miR535-3p in resistance to sheath blight disease in rice |
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| ZHANG Zhaoyang,FENG Tao,ZUO Shimin,OUYANG Shouqiang.The function of Osa-miR535-3p in resistance to sheath blight disease in rice[J].Acta Phytopathologica Sinica,2022,52(5):791-800. |
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| Authors: | ZHANG Zhaoyang FENG Tao ZUO Shimin OUYANG Shouqiang |
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| Institution: | College of Horticulture and Plant Protection, Agricultural College of Yangzhou University, Yangzhou University, Yangzhou 225009, China |
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| Abstract: | Sheath blight is one of the most important diseases in rice, the pathogen Rhizoctonia solani is a typical soil-borne disease. Our previous study found that there were several endogenous miRNAs in response to R. solani infection in rice. Here, our data showed that the expression of Osa-miR535-3p was inhibited by R. solani infection. We constructed knockout and overexpression materials of Osa-miR535-3p, respectively. After infected by R. solani, the disease incidence displayed more serious in Osa-miR535-3p knockout plants compared with the control plants, while the disease incidence was significantly reduced in overexpression plants. In addition, the agronomic traits of transgenic rice have also significantly affected: the overexpression plants of Osa-miR535-3p showed decreased height and secondary branches, shorter panicle length, decreased thousand-grain weight, and increased tillers. While, the knock-out plants of Osa-miR535-3p presented increased secondary branches, increased panicle length, decreased tillers, and increased thousand-grain weight. By RNA-seq, a few genes, including plant hormone signaling pathway related genes, especially, Ethylene(ET)and 3-Indoleacetic acid (IAA) were involved in response to R. solani infection. Taken together, our results suggested that Osa-miR535-3p positively mediated rice immunity against R. solani and impacted on the agronomic traits in rice. |
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| Keywords: | rice sheath blight microRNA sheath blight resistance gene Osa-miR535-3p |
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