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| 十字花科黑腐病菌中一个假定蛋白基因XC_3605与致病相关 | |
| 引用本文： | 王 凛,徐 勇,韦载娲,阳丽艳,杨丽超,姜伯乐.十字花科黑腐病菌中一个假定蛋白基因XC_3605与致病相关[J].植物病理学报,2016,46(2):258-264. |
| 作者姓名： | 王 凛 徐 勇 韦载娲 阳丽艳 杨丽超 姜伯乐 |
| 作者单位： | 广西大学生命科学与技术学院,南宁530004; 桂林医学院,桂林541004; 亚热带农业生物资源保护与利用国家重点实验室,南宁530004 |
| 基金项目： | 国家自然科学基金资助项目(31260443); 广西创新研究团队资助项目(2014GXNSFFA118005); 广西自然科学基金资助项目(2013GXNSFAA019097; 2013GXNSFBA019088) |
| 摘 要： | 十字花科黑腐病菌(Xanthomonas campestris pv.campestris,Xcc)是全球范围内能引起十字花科植物黑腐病的重要植物病原细菌,也是研究植物病原细菌与植物互作机制的模式细菌。利用自杀质粒pK18mobsacB诱变十字花科黑腐病菌Xcc 8004的XC_3605基因,获得缺失突变体D3605。表型分析发现D3605胞外多糖合成能力、游动能力及致病力显著降低,积聚形成生物被膜的能力增强。用带有全长XC_3605基因序列的pLAFRJ对D3605进行功能互补,其胞外多糖产量、游动性、致病力和生物被膜均得到恢复。研究结果表明,XC_3605基因在十字花科黑腐病菌致病过程中发挥作用。 |
| 关 键 词： | 十字花科黑腐病菌 胞外多糖 游动性 致病性 生物被膜 |
A hypothetical gene XC_3605 contributes to pathogenicity of Xanthomonas campestris pv. campestris |
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| WANG Lin,XU Yong,WEI Zai-wa,YANG Li-yan,YANG Li-chao,JIANG Bo-le.A hypothetical gene XC_3605 contributes to pathogenicity of Xanthomonas campestris pv. campestris[J].Acta Phytopathologica Sinica,2016,46(2):258-264. | |
| Authors: | WANG Lin XU Yong WEI Zai-wa YANG Li-yan YANG Li-chao JIANG Bo-le |
| Institution: | College of Life Science and Technology, Guangxi University, Nanning 530004, China; College of Biotechnology, Guilin Medical University, Guilin 541004, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning 530004, China |
| Abstract: | Xanthomonas campestris pv. campestris (Xcc) causes black rot on cruciferous plants and is one of the model bacteria for studying the plant-microbial interaction. The XC_3605 gene of Xcc was deleted by using a suicide plasmid pK18mobsacB to generate the mutant D3605. The exopolysaccharide production, mobility and virulence on the host plant were decreased in mutant D3605, while biofilm formation was stimulated. The altered phenotypes of the mutant could be restored by the intact XC_3605 gene carried in pLAFRJ. These results indicated that XC_3605 gene plays an important role in the pathogenicity of Xcc. |
| Keywords: | Xanthomonas campestris pv campestris exopolysaccharide mobility pathogenicity biofilm |
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