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| 外源水杨酸诱导大麦对条纹病的抗性研究 | |
| 引用本文： | 刘海颖,司二静,郭铭,祁天涛,魏建敏,姚立蓉,汪军成,李葆春,孟亚雄,马小乐,赵池铭,尚勋武,王化俊.外源水杨酸诱导大麦对条纹病的抗性研究[J].植物病理学报,2022,52(4):658-668. |
| 作者姓名： | 刘海颖 司二静 郭铭 祁天涛 魏建敏 姚立蓉 汪军成 李葆春 孟亚雄 马小乐 赵池铭 尚勋武 王化俊 |
| 作者单位： | 甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室, 兰州 730070; 甘肃农业大学农学院, 兰州 730070; 甘肃农业大学生命科学技术学院, 兰州 730070; 甘肃省定西市临洮农业学校, 定西 730500 |
| 基金项目： | 财政部和农业农村部:国家现代农业产业技术体系(CARS-05-04B-2);国家自然科学基金项目(32160496);国家自然科学基金项目(30771331);甘肃省教育厅产业支撑计划项目(2021CYZC-12);甘肃省科技重大专项计划(17ZD2NA016);甘肃省教育厅创新基金项目(2021A-055);甘肃省青年科技基金计划(20JR5RA010);甘肃省科技支撑计划项目(1604NKCA052);甘肃省教育厅创新能力提升项目(2019A-053);甘肃农业大学科技创新基金-公招博士科研启动基金(GSAURCZX201706);甘肃农业大学学科建设专项基金(GAU-XKJS-2018-082、083) |
| 摘 要： | 条纹病是影响大麦生产的主要病害之一,降低大麦条纹病的发生对大麦稳产具有重要意义。水杨酸作为植物内源激素,可以通过激活植物过敏反应和调节植物病程相关蛋白基因表达从而诱导植物产生系统性抗性。本研究以感病大麦品种Alexis为材料,分别采用0、5、10、15和20 mmol·L-1浓度的水杨酸浸种后接种大麦条纹病菌(Pyrenophora graminea),三叶期调查Alexis的发病率、病情指数及生长状况;以10 mmol·L-1浓度的水杨酸处理Alexis种子后人工接种P. graminea,测定不同侵染时间Alexis胚芽的过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)及苯丙氨酸解氨酶(PAL)活性,并检测侵染8 d后Alexis抗病相关基因的相对表达量,旨在明确不同水杨酸浓度对Alexis抗病性的影响及水杨酸诱导Alexis产生抗性的机制。结果表明:0、5、10、15和20 mmol·L-1的水杨酸处理下,Alexis的发病率呈先下降后上升的趋势,病情指数与发病率的变化趋势一致;10 mmol·L-1的水杨酸处理下Alexis的发病率及病情指数较其他浓度处理显著降低;10 mmol·L-1的水杨酸处理下,Alexis的株高、根长、鲜质量及干质量变化显著,分别是0 mmol·L-1水杨酸处理的1.27、1.33、1.27和1.47倍;Alexis萌发2~8 d内,接菌后Alexis胚芽的POD、CAT及SOD活性均高于不接菌处理,PAL活性于第2、4和8 d 时高于不接菌处理;水杨酸接菌处理下Alexis胚芽的POD、CAT、SOD及PAL活性均高于接菌处理;第8 d 水杨酸接菌处理下Alexis抗病相关基因HORVU2Hr1G116090(TGA)和HORVU2Hr1G033620(PR)的相对表达量较接菌处理显著升高,可能参与了Alexis对条纹病菌抗病调控。研究结果可为应用水杨酸防治大麦条纹病提供理论依据。 |
| 关 键 词： | 水杨酸 大麦条纹病 抗病性 防御性酶 抗病相关基因 |
| 收稿时间： | 2021-06-24 |
Resistance of barley to Pyrenophora graminea induced by exogenous salicylic scid |
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| LIU Haiying,SI Erjing,GUO Ming,QI Tiantao,WEI Jianmin,YAO Lirong,WANG Juncheng,LI Baochun,MENG Yaxiong,MA Xiaole,ZHAO Chiming,SHANG Xunwu,WANG Huajun.Resistance of barley to Pyrenophora graminea induced by exogenous salicylic scid[J].Acta Phytopathologica Sinica,2022,52(4):658-668. | |
| Authors: | LIU Haiying SI Erjing GUO Ming QI Tiantao WEI Jianmin YAO Lirong WANG Juncheng LI Baochun MENG Yaxiong MA Xiaole ZHAO Chiming SHANG Xunwu WANG Huajun |
| Institution: | Gansu Provincial Key Lab of Aridland Crop Science / Gansu Provincial Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou 730070, China; College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China; College of life Sciences and Technology, Gansu Agriculture University, Lanzhou 730070, China; Lintao Agricultural School of Dingxi City in Gansu Province,Dingxi 730500, China |
| Abstract: | Barley leaf stripe is one of the main diseases affecting barley production. Reducing the occurrence of barley leaf stripe is of great significance for stable yield of barley. Salicylic acid, as an endogenous hormone in plants, can induce systemic resistance by activating plant hypersensitive response and regulating the gene expression of pathogenesis-related protein. In this study, the susceptible barley variety Alexis was used as mate-rial. The seeds were soaked with salicylic acid at 0, 5, 10, 15 and 20 mmol·L-1 respectively, and then inoculated with Pyrenophora graminea. The incidence and disease index of Alexis were investigated at the three-leaf stage. Alexis seeds were treated with salicylic acid at a concentration of 10 mmol·L-1and inoculated manually to determine the enzymatic activities of peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) and pheny-lalanine ammonialyase (PAL) in Alexis plumule at different infection times and to detect the relative genes expression levels of pathogenesis-related protein in Alexis after 8 d of infection, aiming to clarify the effect of different SA concentrations on Alexis resistance and the resistance mechanism induced by SA in Alexis. The results showed that the incidence of Alexis treated with 0, 5, 10, 15, and 20 mmol·L-1 salicylic acid decreased first and then increased, and the disease index was consistent with the change trend of incidence. The incidence rate and disease index of Alexis treated with 10 mmol·L-1 salicylic acid were significantly lower than those treated with other concentrations. The plant height, root length, fresh weight and dry weight of Alexis treated with 10 mmol·L-1 salicylic acid were significantly changed, which were 1.27, 1.33, 1.27 and 1.47 times higher than those of Alexis treated with 0 mmol·L-1 salicylic acid respectively. The activities of POD, CAT and SOD in the inoculated Alexis plumule were higher than those in the non-inoculated treatment within 2-8 d, and the PAL activity was higher than that in the non-inoculated treatment at 2, 4 and 8 d. The activities of POD, CAT, SOD and PAL of Alexis plumule inoculated with salicylic acid were higher than those of the inoculated trea-tment. The relative genes expression levels of pathogenesis-related protein HORVU2Hr1G116090 (TGA) and HORVU2Hr1G033620 (PR) in Alexis inoculated with salicylic acid on the 8th day were significantly higher than those in the inoculated treatment, suggesting that these genes might be involved in the regulation of Alexis resis-tance to P. graminea. The results could provide a theoretical basis for the application of salicylic acid to control barley leaf stripe. |
| Keywords: | salicylic acid barley leaf stripe disease resistance defensive enzyme genes related to disease resistance |
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