| 小麦叶锈菌生理小种MFR的分子鉴定研究 |
| |
| 引用本文： | 蒲志刚,刘太国,张敏,陈万权.小麦叶锈菌生理小种MFR的分子鉴定研究[J].植物病理学报,2004,34(5):449-454. |
| |
| 作者姓名： | 蒲志刚 刘太国 张敏 陈万权 |
| |
| 作者单位： | 1 中国农业科学院植物保护研究所, 北京 100094;2 四川农业大学农学院, 雅安 625014 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| |
| 摘 要： | 用AFLP方法对来自中国和墨西哥的23个小麦叶锈菌生理小种进行分析,共筛选了64对引物,获得一对引物(M05/E03)可在MFR小种中扩增出一条特异性DNA片段,进行回收、克隆、测序,结果表明该片段具有325个碱基。根据特异性片段序列设计出SCAR标记引物,对60个叶锈菌生理小种分离物进行回检结果表明,研制的SCAR标记能够准确区分MFR生理小种。本实验结果为小麦锈菌生理小种分子检测体系的建立奠定了基础
|
| 关 键 词： | 小麦叶锈菌 分子标记 毒性 AFLP |
| 文章编号： | 0412-0914(2004)05-0449-06 |
| 修稿时间： | 2004年3月23日 |
Development of a molecular detection assay for the physiological race MFR of Puccinia triticina |
| |
| PU Zhi gang ,LIU Tai guo ,ZHANG Min ,CHEN Wan quan.Development of a molecular detection assay for the physiological race MFR of Puccinia triticina[J].Acta Phytopathologica Sinica,2004,34(5):449-454. |
| |
| Authors: | PU Zhi gang LIU Tai guo ZHANG Min CHEN Wan quan |
| |
| Institution: | 1 Institute of Plant Protection, CAAS, Beijing 100094, China;2 College of Agronomy, Sichuan Agricultural University, Yaan 625014, China |
| |
| Abstract: | A total of twenty three physiological races of Puccinia triticina from wheat growing areas of China and Mexico were investigated for the DNA polymorphism by amplified fragment length polymorphism (AFLP). Sixty four pairs of AFLP primers were used to generate polymorphic bands, and one specific DNA fragment was amplified by M 05 /E 03 primer combination in race MFR of Puccinia triticina . The specific DNA polymorphic fragment, a 325 base pair band, was excised and cloned into T easy vector. Based on the cloning and sequencing, the AFLP marker was converted to a sequence characterized amplified region (SCAR) marker. Sixty isolates of Puccinia triticina were tested with the SCAR marker,indicating that MFR race could be differentiated from the rest races by the specific DNA marker exactly. The results provide a useful aid to establish the system of molecular detection for the physiological races of wheat rust fungi. |
| |
| Keywords: | AFLP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《植物病理学报》浏览原始摘要信息 |
| 点击此处可从《植物病理学报》下载免费的PDF全文 |
|
