| 水稻纹枯病寄主-病原物互作鉴别品种与菌株的筛选 |
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| 引用本文： | 陈夕军,王玲,左示敏,王子斌,陈宗祥,张亚芳,鲁国东,周而勋,郭泽建,黄世文,潘学彪.水稻纹枯病寄主-病原物互作鉴别品种与菌株的筛选[J].植物病理学报,2009,39(5):514-520. |
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| 作者姓名： | 陈夕军 王玲 左示敏 王子斌 陈宗祥 张亚芳 鲁国东 周而勋 郭泽建 黄世文 潘学彪 |
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| 作者单位： | 1 扬州大学江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室, 扬州 225009;2 中国水稻研究所, 杭州 310006;3 福建农林大学, 福州 350002;4 华南农业大学, 广州 510642;5 中国农业大学, 北京 100193 |
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| 基金项目： | 农业公益性行业专项，国家863高新技术研究发展项目，扬州大学校基金项目 |
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| 摘 要： | 经过近十年的病原菌接种试验,从国内外数百份水稻种质中,筛选出了抗病水平不同的5个代表品种Lemont、武育粳3号、Jasmine85、C418和YSBR1,作为水稻纹枯病菌致病力的鉴别品种。从江苏、广东、广西、海南、云南、湖南、福建等省采集、分离的水稻纹枯病菌中,初步筛选出致病力有差异的30个菌株,于温室苗期接种上述5个品种,确定了不同致病力的5个代表菌株C30、GD-118、E67、YN-7和YN-3。将选出的品种和菌株在江苏扬州和浙江富阳进行大田成株期抗性和致病力分析,结果表明:品种的抗病性和菌株的致病力都存在极显著差异,分别属于5个和3个不同的显著性级别,均可暂时作为鉴定材料使用。据以上结果,初步建立了一套可供水稻纹枯病菌致病力检测和寄主抗病性鉴定的鉴别体系。本文还讨论了建立水稻纹枯病鉴别体系的必要性、可行性和需要进一步开展的工作。
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| 关 键 词： | 水稻纹枯病 抗性鉴定 鉴别品种 鉴别菌株 筛选 |
Screening of varieties and isolates for identifying interaction between host and pathogen of rice sheath blight |
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| CHEN Xi-jun,WANG Ling,ZUO Shi-min,WANG Zi-bin,CHEN Zong-xiang,ZHANG Ya-fang,LU Guo-dong,ZHOU Er-xun,GUO Ze-jian,HUANG Shi-wen,PAN Xue-biao.Screening of varieties and isolates for identifying interaction between host and pathogen of rice sheath blight[J].Acta Phytopathologica Sinica,2009,39(5):514-520. |
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| Authors: | CHEN Xi-jun WANG Ling ZUO Shi-min WANG Zi-bin CHEN Zong-xiang ZHANG Ya-fang LU Guo-dong ZHOU Er-xun GUO Ze-jian HUANG Shi-wen PAN Xue-biao |
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| Institution: | 1 Key Laboratory of Plant Functional Genornics of Ministry of Education/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China;2 China National Rice Research Institute, Hangzhou 310006, China;3 Fujian Agricultural and Forestry University, Fuzhou 350002, China;4 Southern China Agricultural University, Guangzhou 510642, China;5 China Agricultural University, Beijing 100193, China |
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| Abstract: | Five rice cultivars (YSBR1 ,C418,Jasmine85,Wuyujing3 and Lemont) with different levels of resistance to Rhizoctonia solani identified and screened from worldwide rice collections were selected to identify the pathogenicity of different isolates. Several hundred strains of R. solani had been isolated and purified from Jiangsu,Guangdong,Guangxi,Hainan, Yunnan,Hunan and Fujian Province. Thirty isolates with different path-ogenicity were selected to inoculate the five identification cultivars at the seedling stage in the greenhouse. Five isolates with obvious discrepancy of pathogenicity, C30,GD-118,E67, YN-7 and YN-3, were selected ulti-mately. The obtained cultivars and strains for identification were verified through field experiments at rice adult stage in Yangzhou of Jiangsu Province and Fuyang of Zhejiang Province. The results indicated that both the cultivars and isolates were divided significantly to five resistance grades and three pathogenicity grades respectively, so both of which could be used as identification materials. An identification system to the isolate pathogenity and the host resistance for rice sheath blight was preliminarily setup. The necessity and feasibility about the identification system, and the further work were also discussed in the paper. |
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| Keywords: | rice sheath blight resistance identification identification varieties identification isolates screening |
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