| 广东果树上17种拟茎点霉的RAPD分析 |
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| 引用本文： | 姜子德,梅曼彤,戚佩坤.广东果树上17种拟茎点霉的RAPD分析[J].植物病理学报,2003,33(1):35-39. |
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| 作者姓名： | 姜子德 梅曼彤 戚佩坤 |
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| 作者单位： | 1 华南农业大学热带亚热带真菌研究室;2 华南农业大学遗传工程研究室, 广州 510642 |
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| 基金项目： | 国家自然科学基金资助项目 (39870 0 0 1、3989940 0 )，《高等学校骨干教师资助计划》项目 |
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| 摘 要： | 自320个随机引物中筛选出适于拟茎点霉属真菌种间亲缘关系分析的15个随机引物,并优化了RAPD分析的扩增体系,在此基础上,对广东果树上17种拟茎点霉进行了RAPD分析。各菌株间的Nei相似系数UPGMA法聚类结果表明:来源于不同地区的2个Phomopsis mangiferae Ahmad菌株和2个P.macadami Z.D.Jiang et P.K.Chi菌株都分别以0.636和0.589的相似系数两两首先聚在一起,而不同的种则只在小于0.54的相似系数范围内聚类,体现了种间及种内的亲缘关系差异程度;聚类群与寄主植物不具相关性,同种植物上的不同拟茎点霉,即使是分自相同寄生部位也不能聚在一类;支持形态学上将生于柑桔枝和黄皮茎、沙梨叶和果、杨梅叶和枝以及同是生于龙眼叶的共8个拟茎点霉分别鉴定为不同的种,而不支持将P. cytosporella Penz.et Sacc.与P. mangiferae合并为一个种的观点;RAPD技术可作为拟茎点霉属真菌种间的亲缘关系分析的重要手段。
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| 关 键 词： | 拟茎点霉 亲缘关系 随机扩增多态性DNA 果树 |
| 文章编号： | 0412-0914(2003)01-0035-05 |
| 修稿时间： | 2001年9月12日 |
RAPD analysis of seventeen species of Phomopsis on fruit trees in Guangdong |
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| JIANG Zi-de ,MEI Man-tong ,QI Pei-kun.RAPD analysis of seventeen species of Phomopsis on fruit trees in Guangdong[J].Acta Phytopathologica Sinica,2003,33(1):35-39. |
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| Authors: | JIANG Zi-de MEI Man-tong QI Pei-kun |
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| Institution: | 1 Lab of Tropical and Subtropical Fungi of South China Agric. Univ., Guangzhou 510642, China;2 Lab of Genetic Engineering of South China Agric. Univ., Guangzhou 510642, China |
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| Abstract: | To study phylogenetic relationships among 19 isolates of 17 species of Phomopsis on fruit trees in Guangdong Province, 15 suitable primers selected from 320 random primers screened were used for RAPD analysis. According to the phylogenetic tree constructed by UPGMA of Nei's similarity coefficients among the tested isolates based on the RAPD data, 2 isolates of Phomopsis mangiferae Ahmad and 2 isolates of P. macadami Z.D.Jiang et P.K.Chi collected from different areas could be clustered into one group with similarity of 63.6% and 58.9%, respectively. However, the similarity coefficients for different species were less than 54%, which indicated there were various differences among interspecies and intraspecies. No correlation was found between the groups and hosts of the isolates and different species could not be clustered into one group from the same host, even from the same part of a host. The RAPD data also supported the results from morphological identification, in which 8 isolates from wampee stem and citrus branch, Chinese bayberry leaf and branch, sand pear leaf and fruit and longan leaf could be identified to be independent of each other, but did not support the suggestion that P. mangiferae can be combined into P. cytosporella Penz. et Sacc. It is concluded from this research that RAPD technique can be used to analyze phylogenetic relationship of Phomopsis. |
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| Keywords: | Phomopsis phylogenetic relation RAPD fruit tree |
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