| 灰葡萄孢对氟唑菌酰羟胺不同敏感型菌株的生物学特性 |
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| 引用本文： | 杨可心,毕秋艳,吴杰,路粉,王文桥,韩秀英,赵建江.灰葡萄孢对氟唑菌酰羟胺不同敏感型菌株的生物学特性[J].农药学学报,2022,24(4):743-751. |
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| 作者姓名： | 杨可心 毕秋艳 吴杰 路粉 王文桥 韩秀英 赵建江 |
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| 作者单位： | 1.河北省农林科学院 植物保护研究所，河北省农业有害生物综合防治工程技术研究中心，农业农村部华北北部作物有害生物综合治理重点实验室，河北 保定 071000 |
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| 基金项目： | 河北省自然科学基金(C2019301076)；河北省重点研发计划(21326510D). |
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| 摘 要： | 为明确灰葡萄孢对氟唑菌酰羟胺不同敏感型菌株的生物学特性差异，将9个不同敏感型菌株在无药PDA平板上继代培养10代后，测定其对氟唑菌酰羟胺的敏感性。采用菌丝生长速率法分别在PDA平板和离体叶片上测定了其菌丝生长速率、产孢量、孢子萌发率和致病性，以及其对温度、pH值和葡萄糖的敏感性，并对其编码琥珀酸脱氢酶的SdhA、SdhB、SdhC和SdhD 4个亚基基因进行了克隆与测序。结果显示:从田间获得的2个低抗菌株、2个中抗菌株和2个高抗菌株对氟唑菌酰羟胺的敏感性变异指数在0.60~1.15之间，而通过室内药剂驯化获得的高抗菌株BLH5F对氟唑菌酰羟胺的敏感性变异指数为0.23；除低抗菌株WP8的菌丝生长速率显著低于2个敏感菌株，以及中抗菌株WP6和高抗菌株WPE9的产孢量显著低于2个敏感菌株外，其余各抗性菌株在菌丝生长速率、产孢量、孢子萌发率和致病性方面均无显著差异。整体而言，7个抗性菌株在对温度、pH值和葡萄糖的敏感性方面与敏感菌株无显著差异。基因克隆及测序结果表明，室内药剂驯化获得的高抗菌株BLH5F SdhB基因的272位由组氨酸变为了精氨酸 (H272R)，田间获得的低抗、中抗和高抗各2个菌株均为SdhB基因的225位由脯氨酸变为了亮氨酸 (P225L)。综合分析表明，灰葡萄孢对氟唑菌酰羟胺不同敏感型菌株具有相似的适合度。因此，推测在药剂的选择压下，抗氟唑菌酰羟胺的灰葡萄孢菌株在田间容易形成优势种群。
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| 关 键 词： | 灰葡萄孢 氟唑菌酰羟胺 抗性菌株 敏感性 生物学特性 SdhB基因 |
| 收稿时间： | 2021-12-13 |
Biological characterization of Botrytis cinerea isolates with different sensitivity types to pydiflumetofen |
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| Institution: | 1.Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, IPM Center of Hebei Province, Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, Baoding 071000, Hebei Province, China |
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| Abstract: | To determine the biological characteristics of Botrytis cinerea with different sensitivity types to pydiflumetofen, the resistance stability of nine B. cinerea isolates with different sensitivity types to pydiflumetofen was determined by the mycelial growth method, after sub-cultivation on fungicide-free PDA plates for ten generations, the biological characteristics such as mycelial growth rate, conidial production, conidium germination rate, and pathogenicity of these nine isolates were determined on PDA medium and detached leaves; The sensitivity of these nine isolates to temperature, pH value, and glucose was determined by mycelial growth method, and the four succinate dehydrogenase subunits gene A(SdhA), B(SdhB), C(SdhC) and D(SdhD) were cloned and sequenced. These results showed that the factors of sensitivity change (FSC) of two low-resistant isolates, two medium-resistant isolates, and two high-resistant isolates obtained from the field ranged from 0.60 to 1.15, while the FSC of the high-resistant isolate BLH5F obtained by fungicide-selection in the laboratory was 0.23. There was no significant difference between resistant and sensitive phenotypes of the B. cinerea isolates on mycelial growth, spore production, conidium germination rate, and pathogenicity, except that the mycelial growth rate of the low-resistant isolate WP8 was significantly lower than that of the two sensitive isolates, and the spore productions of the medium-resistant isolate WP6 and the high-resistant isolate WPE9 were significantly lower than that of the two sensitive isolates. On the whole, there was no significant difference between the seven resistant isolates and the two sensitive isolates in the sensitivity to temperature, pH value, and glucose. The histidine (His) was replaced by arginine (Arg) at position 272 of SdhB gene (H272R) in the high resistance isolate BLH5F obtained from the laboratory by fungicide-selection and the proline (Pro) changed to leucine (Leu) at position 225 of SdhB gene (P225L) in two low-resistant isolates, two medium-resistant isolates, and two high-resistant isolates obtained from the field. All the results showed that the fitness of B. cinerea with different sensitivity types to pydiflumetofen was similar. So, it was speculated that the isolates resistant to pydiflumetofen were easy to form a dominant population under the fungicide selection in the field. |
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