| 侵染牡丹的烟草脆裂病毒的检测鉴定及序列分析 |
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| 引用本文： | 贺振,陈春峰,罗云建,陈夕军.侵染牡丹的烟草脆裂病毒的检测鉴定及序列分析[J].植物保护学报,2018,45(4):893-899. |
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| 作者姓名： | 贺振 陈春峰 罗云建 陈夕军 |
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| 作者单位： | 扬州大学园艺与植物保护学院 |
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| 基金项目： | 国家自然科学基金（31601604，31500388），江苏省高校自然科学基金（16KJB210015） |
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| 摘 要： | 为明确江苏省牡丹上烟草脆裂病毒(Tobacco rattle virus,TRV)的发生情况,利用ELISA和RT-PCR方法对采集自扬州市的40份牡丹叶片样品进行了检测鉴定,测定了其中一个分离物Peony-11中TRV RNA1分子的部分蛋白编码区序列,并结合Gen Bank中已报道的相关序列对其进行了多样性和系统发生分析。结果显示,江苏省扬州市牡丹上TRV的检出率高达62.5%;序列分析表明本研究获得的TRV牡丹分离物Peony-11与Gen Bank中其它63个分离物的核苷酸序列一致率为90.5%~99.7%;系统发生和遗传距离分析表明TRV可以分成2个组10个亚组,组间、亚组间具有较为清晰的地理和寄主特异性,其中Peony-11分离物位于亚组I-1。
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| 关 键 词： | 牡丹 烟草脆裂病毒 序列分析 系统发生分析 |
| 收稿时间： | 2017/3/1 0:00:00 |
Molecular detection, identification and genetic diversity of Tobacco rattle virus on peony |
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| He Zhen,Chen Chunfeng,Luo Yunjian and Chen Xijun.Molecular detection, identification and genetic diversity of Tobacco rattle virus on peony[J].Acta Phytophylacica Sinica,2018,45(4):893-899. |
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| Authors: | He Zhen Chen Chunfeng Luo Yunjian and Chen Xijun |
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| Institution: | School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu Province, China,School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu Province, China,School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu Province, China and School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu Province, China |
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| Abstract: | In order to investigate the occurrence of Tobacco rattle virus (TRV) in peony in Jiangsu Province, 40 leaf samples collected from Yangzhou were detected by ELISA and RT-PCR. Partial ORF1 coding region of TRV RNA1 (Peony-11) was sequenced and analyzed with related sequences on GenBank. According to the results, the detection rate of TRV in peony was 62.5% in Yangzhou. Sequence analysis indicated that the TRV peony isolate determined in the present study shared nucleotide identities of 90.5%-99.7% with 63 TRV isolates downloaded from GenBank. Phylogenetic and genetic distance analysis demonstrated that TRVs were clustered into two groups, ten subgroups with clear geography and host specificity, while the TRV Peony-11 isolate determined here belonged to subgroup I-1. |
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| Keywords: | peony Tobacco rattle virus sequence analysis phylogenetic analysis |
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