| 实时荧光定量PCR在植物害虫研究中的应用 |
| |
| 引用本文： | 郭庆,王忠跃,孔繁芳,武艳霞.实时荧光定量PCR在植物害虫研究中的应用[J].江西植保,2011,34(2):47-53. |
| |
| 作者姓名： | 郭庆 王忠跃 孔繁芳 武艳霞 |
| |
| 作者单位： | 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,100193 |
| |
| 摘 要： | 实时荧光定量PCR技术作为一项新兴技术,以快速、准确定量、便捷等优点广泛应用于科学研究各个领域,越来越受到人们的重视。近年来,随着实时荧光定量PCR技术的发展和深入,大大提高了植物害虫的研究水平。本文结合了最近几年来应用于鉴定和研究植物害虫的情况,同时也介绍了在植物病原线虫和害螨研究中的应用,综合论述了实时荧光定量PCR技术在植物害虫研究中的应用。
|
| 关 键 词： | 实时荧光定量PCR Ct 植物害虫 荧光阈值 荧光探针 |
Application of Real-time Fluorescence Quantitative PCR on Plant Pests |
| |
| GUO Qing,WANG Zhong-yue,KONG Fan-fang,WU Yan-xia.Application of Real-time Fluorescence Quantitative PCR on Plant Pests[J].Jiangxi Plant Protection,2011,34(2):47-53. |
| |
| Authors: | GUO Qing WANG Zhong-yue KONG Fan-fang WU Yan-xia |
| |
| Institution: | (State Key Laboratory for Biology of Plant Diseases and Insect pests,Institute of Plant Protection, CAAS,Beijing 100193,P.R.China) |
| |
| Abstract: | As an emerging technology,it has been widely used in different research areas,since it is fast,accurate,and easy use,more and more people pay attention to the real-time fluorescence quantitative PCR.In recent years,with the developing and more detailed of the technology,RQ PCR has greatly improved the level of research on plant pests.In this review,it was summarized and introduced application of real-time fluorescence quantitative PCR on insect pests,pathogenic nematodes and mites. |
| |
| Keywords: | real-time fluorescence quantitative PCR Ct plant pests cycle of threshold fluorescent probe |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
