| 三种抗原检测H1N1猪流感病毒抗体的比较 |
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| 引用本文： | 杨倩,薛春宜,朱道中,李晓明,曹永长.三种抗原检测H1N1猪流感病毒抗体的比较[J].广东畜牧兽医科技,2014(1):16-20. |
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| 作者姓名： | 杨倩 薛春宜 朱道中 李晓明 曹永长 |
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| 作者单位： | [1] 中山大学生命科学学院有害生物控制与资源利用国家重点实验室,广东广州510006 [2] 广东出入境检验检疫局检验检疫技术中心,广东广州510623 |
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| 基金项目： | 基金项目：国家质检总局科技计划项目（2011IK004） |
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| 摘 要： | 本研究采用昆虫杆状病毒表达系统制备了H1N1亚型猪流感病毒的HA蛋白、类病毒脂质体、病毒样颗粒,分别作为抗原进行包被,采用间接酶联免疫吸附实验（ELISA）检测H1N1猪流感病毒抗体.特异性实验结果表明,单独表达的HA蛋白和类病毒脂质体特异性良好,但是病毒样颗粒不能区分不同亚型的流感病毒;敏感性实验结果表明,类病毒脂质体作为抗原的敏感性最好;重复性实验结果表明,三种抗原的重复性好,ELISA实验的批间和批内变异系数均小于10％;稳定性实验结果表明,在4℃可至少稳定保存1年.综上所述,类病毒脂质体有较高的免疫反应性,与灭活全病毒相比有更好的安全性,在抗体水平测定方面有较好的应用前景.
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| 关 键 词： | 昆虫杆状病毒表达系统 猪流感病毒 类病毒脂质体 抗体检测 |
Comparation of Three Methods for Detection of Antibodies to H1N1 Swine Influenza Virus |
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| Yang Qian,Xue Chunyi,Zhu Daozhong,Li Xiaoming,Cao Yongchang.Comparation of Three Methods for Detection of Antibodies to H1N1 Swine Influenza Virus[J].Guangdong Journal of Animal and Veterinary Science,2014(1):16-20. |
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| Authors: | Yang Qian Xue Chunyi Zhu Daozhong Li Xiaoming Cao Yongchang |
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| Institution: | 1.State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006,China;2. Technical Center,Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou,510623,China ) |
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| Abstract: | HA protein, virus-like liposomes, and virus-like particles, expressed by Bac-to-Bac baculovirus expression system, were coated as antigens to detect the H1N1 swine influenza virus antibody using indirect enzyme-linked immunosorbent assay （ELISA）. The results of specificity experiments showed that the single-expressed HA protein and virus-like liposomes had good specificity, but the virus-like particles could not distinguish different subtypes of influenza virus. The results of sensitivity experiments showed that virus-like liposomes had the best sensitivity. The results of repetitive experiments showed that the repetitiveness of the three methods was high, and the coefficient of variation within-rim and between-run were less than 1%. The results of stability experiments showed that three antigens could be stable at 4 ~C for at least one year. In a word, the virus-like liposome has strongest immunoreactivity, and is safer than the inactivated whole influenza virus. Thus it represents a promising method in detection of H1N1 antibodies. |
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| Keywords: | Bac-to-Bac baculovirus expression system swine influenza virus virus-like liposome antibody detection |
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