| 桑叶提取物的抗病毒活性检测 |
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| 引用本文： | 王辉,吴莎,孙毅凡,江振友,廖森泰,刘凡.桑叶提取物的抗病毒活性检测[J].蚕业科学,2011,37(5):952-956. |
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| 作者姓名： | 王辉 吴莎 孙毅凡 江振友 廖森泰 刘凡 |
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| 作者单位： | 暨南大学医学院微生物与免疫学系,广州,510632;广东省农业科学院蚕业与农产品加工研究所,广州,510610 |
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| 基金项目： | 广东省自然科学基金团队项目(No.9351064001000002) |
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| 摘 要： | 桑叶作为中药或功能饮品的重要成分在民间和临床上广泛应用。用70%乙醇对广东桑、白桑、鸡桑、鲁桑等桑种共20个品种的桑叶进行浸提,并依次用石油醚、乙酸乙酯、正丁醇萃取得到不同萃取组分及剩余水相组分。利用细胞病变减少法(CPE法)检测发现多个桑叶样品的萃取组分或水相组分含有抗病毒活性物质,且抗病毒活性具有选择性。其中,能有效抑制单纯疱疹病毒1型(HSV-1)的样品最多,6个桑品种桑叶的乙酸乙酯萃取组分对HSV-1的半数抑制浓度(IC50)≤12.5μg/mL;广东桑品种桑叶的水相组分和石油醚萃取组分具有较高的抗甲型流感病毒(Flu A)活性;部分桑品种桑叶的萃取组分对登革病毒2型(DV2)也有一定的抑制活性;20个桑品种桑叶的4种萃取组分对柯萨奇B3病毒(CVB3)均无抑制活性。上述结果表明桑叶含有丰富、较为广谱的抗病毒活性成分,但不同桑品种间的桑叶化学成分及抗病毒活性有一定差异。
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| 关 键 词： | 桑叶 萃取组分 抗病毒活性 桑品种 |
An Assay on the Antiviral Activity of Mulberry Leaf Extract |
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| WANG Hui,WU Sha,SUN Yi-Fan,JIANG Zhen-You,LIAO Sen-Tai,LIU Fan.An Assay on the Antiviral Activity of Mulberry Leaf Extract[J].Acta Sericologica Sinica,2011,37(5):952-956. |
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| Authors: | WANG Hui WU Sha SUN Yi-Fan JIANG Zhen-You LIAO Sen-Tai LIU Fan |
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| Institution: | WANG Hui1 WU Sha1 SUN Yi-Fan1 JIANG Zhen-You1 LIAO Sen-Tai2 LIU Fan2(1Department of Microbiology and Immunology,School of Medicine,Jinan University,Guangzhou 510632,China,2Sericulture & Farm Produce Processing Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510610,China) |
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| Abstract: | Mulberry leaves are widely used in clinic and in public as an important ingredient in traditional Chinese medicine and functional drinks.In this study,mulberry leaves of 20 varieties from Morus atropurpurea Roxb.,Morus alba L.,Morus australis Poir.,and Morus multicaulis Perr.were extracted with 70% ethanol first and then the crude products were further extracted in order of petroleum ether,ethyl acetate and n-butyl alcohol to obtain the correspondent extracted fraction and residue water fraction.Cytopathic ... |
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| Keywords: | Mulberry leaf Extracted fraction Antivrial activity Mulberry variety |
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