| 应用16S rRNA基因序列鉴定柞蚕空胴病病原菌 |
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| 引用本文： | 商翠芳,秦利,赵振军,宋策,李树英,姜德富,范琦.应用16S rRNA基因序列鉴定柞蚕空胴病病原菌[J].蚕业科学,2011,37(5):931-936. |
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| 作者姓名： | 商翠芳 秦利 赵振军 宋策 李树英 姜德富 范琦 |
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| 作者单位： | 沈阳农业大学生物科学技术学院,沈阳,110866;辽宁省农业科学院大连生物技术研究所,辽宁大连,116024;辽宁省蚕业科学研究所,辽宁凤城,118100 |
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| 基金项目： | 现代农业产业技术体系专项(No.CARS-22) |
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| 摘 要： | 20世纪70年代末,采用形态分类学方法,将引起柞蚕空胴病的致病菌鉴定为柞蚕链球菌(Streptococcus pernyi sp.nov)。分别提取已分离柞蚕空胴病的5株病原菌株的基因组DNA,PCR扩增16S rRNA基因片段,经克隆、测序后,与GenBank中登录的相关肠球菌、链球菌菌株的16S rRNA基因序列进行同源性比对并构建系统进化树。结果表明,供试的5株菌株的16S rRNA基因序列相似性在99.5%～99.9%之间,相互之间存在着10个可变位点,推测5株菌株属于同一个菌种;5株菌株的16S rRNA基因序列与肠球菌属(Enterococcus)16S rRNA基因序列的相似性较高,在92.4%～99.8%之间,而与链球菌属(Streptococcus)16S rRNA基因序列的相似性相对较低,在87.3%～87.8%之间;5株菌株与肠球菌属在系统进化树上聚为一类。基于菌株的16S rRNA基因序列分析,鉴定柞蚕空胴病的病原菌应归属于肠球菌属。
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| 关 键 词： | 柞蚕空胴病 16S rRNA基因 肠球菌属 |
Identification of the Pathogen for Antheraea pernyi Empty-gut Disease by Using 16S rRNA Gene Sequence |
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| SHANG Cui-Fang,QIN Li,ZHAO Zhen-Jun,Song Ce,LI Shu-Ying,JIANG De-Fu,FAN Qi.Identification of the Pathogen for Antheraea pernyi Empty-gut Disease by Using 16S rRNA Gene Sequence[J].Acta Sericologica Sinica,2011,37(5):931-936. |
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| Authors: | SHANG Cui-Fang QIN Li ZHAO Zhen-Jun Song Ce LI Shu-Ying JIANG De-Fu FAN Qi |
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| Institution: | SHANG Cui-Fang1 QIN Li1 ZHAO Zhen-Jun3 Song Ce2 LI Shu-Ying3 JIANG De-Fu2* FAN Qi3*(1College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang 110866,China,2The Sericultural Research Institute of Liaoning Province,Fengcheng Liaoning 118100,3Dalian Institute of Biotechnology,Liaoning Academy of Agricultural Sciences,Dalian Liaoning 116024,China) |
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| Abstract: | The pathogen causing empty-gut disease in Antheraea pernyi was identified as Streptococcus pernyi sp.nov by the end of 1970s according to its morphological characters.In this study,we isolated genomic DNAs from 5 different isolates of pathogen causing empty-gut disease in Antheraea perny and amplified their 16S rRNA gene fragments by PCR.After cloning and sequencing,the 16S rRNA gene sequences were compared with those from other strains of Enterococcus and Streptococcus registered in GenBank based on which ... |
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| Keywords: | Antheraea pernyi empty-gut disease 16S rRNA gene Enterococcus |
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