| 家蚕病原白僵菌原生质体分离条件的研究 |
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| 引用本文： | 时连根.家蚕病原白僵菌原生质体分离条件的研究[J].蚕业科学,1997,23(2):100-103. |
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| 作者姓名： | 时连根 |
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| 作者单位： | 浙江农业大学蚕学系 |
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| 摘 要： | 调查研究了家蚕病原白僵菌（Beauveriabassiana）原生质体的分离制备条件。以6mg／mLDriselase液为酶解液，以0.7mol／LNaCl液（pH5．8）为渗透压稳定剂，在30℃下轻轻振荡处理1．5h，能从家蚕病原白但菌嫩菌丝中分离出2～4×107个／mL原生质体，这是家蚕病原白鹰菌原生质体的最适分离条件。
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| 关 键 词： | 家蚕 白僵菌 原生质体 分离条件 |
STUDIES ON ISOLATION CONDITIONS OF PROTOPLASTS FRIM Beauveria bassiana,A PATHOGENIC FUNGUS TO Bombyx mori L. |
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| Shi Liangen.STUDIES ON ISOLATION CONDITIONS OF PROTOPLASTS FRIM Beauveria bassiana,A PATHOGENIC FUNGUS TO Bombyx mori L.[J].Acta Sericologica Sinica,1997,23(2):100-103. |
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| Authors: | Shi Liangen |
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| Abstract: | Conditions for isolation of protoplasts from pathogenic fungus, Beauveria bassianaof silkworm, Bombyx mori L. were studied. A high yield of 2-4 × 107 protoplasts permilliliter from young mycelia of B. bassiana was obtained by gentle shaking treatmentsat 30℃ for 1. 5 hours with Driselase (6mg/mL) as enzyme solution, 0. 7mol/L NaCl(pH5. 8)as osmotic stabilizer. The above treatments were concluded to be the most suit-able conditions for isolating protoplasts from B. bassiana. |
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| Keywords: | Bombyx mori Beauveria bassiana Protoplast Isolation condition |
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