| 广州桑树植原体分子检测及多样性初探 |
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| 引用本文： | 刘清神,许东林,林盘芳,周国辉.广州桑树植原体分子检测及多样性初探[J].蚕业科学,2006,32(1):1-5. |
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| 作者姓名： | 刘清神 许东林 林盘芳 周国辉 |
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| 作者单位： | 华南农业大学动物科学学院,广州,510642;华南农业大学资源环境学院,广州,510642 |
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| 基金项目： | 广东省科技攻关项目(编号2003B21604) |
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| 摘 要： | 采用植原体16S-23S rDNA区段的通用引物对P1/P7和巢式引物对Rm16F2/Rm16R1,建立了快速准确的桑树植原体巢式PCR检测技术。对广州的两个桑树品种资源圃中的部分桑树品种进行了植原体分子检测,结果在两个资源圃中均发现有植原体存在。对巢式PCR的扩增产物(16S rDNA片段)进行了限制性片断长度多态性(RFLP)分析,显示出3种RFLP带型,暗示桑树植原体存在多样性。对所得植原体16S rDNA片段进行序列测定,并与其它植物植原体作亲缘关系分析,结果表明该植原体的16S rDNA序列与其它植物病原植原体之间的同源性为83.3%~99.9%,并初步判断所检测到的桑树植原体属于16S rI组。
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| 关 键 词： | 桑树 植原体 16S rDNA 分子检测 多样性 |
| 文章编号： | 0257-4799(2006)01-0001-05 |
| 收稿时间： | 2005-08-22 |
| 修稿时间： | 2005年8月22日 |
Molecular Detection of Mulberry Phytoplasma in Guangzhou and Primary Study of its 16S rDNA Diversity |
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| LIU Qing-Shen,XU Dong-Lin,LIN Pan-Fang,ZHOU Guo-Hui.Molecular Detection of Mulberry Phytoplasma in Guangzhou and Primary Study of its 16S rDNA Diversity[J].Acta Sericologica Sinica,2006,32(1):1-5. |
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| Authors: | LIU Qing-Shen XU Dong-Lin LIN Pan-Fang ZHOU Guo-Hui |
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| Institution: | 1 College of Animal Science, South China Agricultural University, Guangzhou 510642, China; 2 College of Besources and Environment, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | Based on the 16S-23S rDNA sequence of phytoplasma,a set of universal primers P1/P7 and a set of nested primers Rm16F2/Rm16R1 were employed to perform nested-PCR detection for pathogenic phytoplasma in mulberry leaf tissues sampled from two variety-collecting bases in Guangzhou,China.It was confirmed that some mulberry varieties in these bases were phytoplasma-infected.The products of nested-PCR,16S rDNA fragments,were then analyzed by restricted fragment length polymorphism with KpnⅠ and MspⅠ and by 6% polyacylamide gel electrophoresis.The result showed that there were three types of RFLP bands which indicated the diversity of mulberry phytoplasma.The obtained 16S rDNA fragments were sequenced and then compared with the reported phytoplasma 16S rDNA.The nucleotide identities were from 83.3% to 99.9% between the detected phytoplasma and others.According to 16S rDNA sequence the phylogenetic tree was constructed.Based on the above results,we deduced that the phytoplasma causes mulberry dwarf disease in Guangzhou belongs to group 16S rⅠ. |
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| Keywords: | Mulberry Phytoplasma 16S rDNA Molecular detection Diversity |
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