| 利用SSR标记对家蚕耐氟基因进行连锁定位分析 |
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| 引用本文： | 白会钗,徐安英,李木旺,赵云坡,郭秋红,钱荷英,张月华,赵远,黄勇平,郭锡杰.利用SSR标记对家蚕耐氟基因进行连锁定位分析[J].蚕业科学,2008,34(2):191-196. |
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| 作者姓名： | 白会钗 徐安英 李木旺 赵云坡 郭秋红 钱荷英 张月华 赵远 黄勇平 郭锡杰 |
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| 作者单位： | 江苏科技大学,江苏,镇江,212003;中国农业科学院蚕业研究所,江苏,镇江,212018;中国科学院上海植物生理生态研究所,上海,200032 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)
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江苏省高技术研究计划(农业)项目 |
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| 摘 要： | 采用家蚕耐氟品系T6和敏感品系733新组配正反交群体(733新×T6)×733新和733新×(733新×T6),分别记作BC1F和BC1M。基于雌性家蚕染色体不发生交换,用已构建的家蚕SSR连锁图上的标记对耐氟基因(dominant endurangce to fluoride,Def)进行了定位及连锁分析。在28个连锁群上都找到了多态标记,其中只有3个位于12连锁群上的SSR标记与耐氟基因连锁。根据第12连锁群上已有的微卫星序列,寻找其所在的Scaffold上的其它SSR位点,并设计引物,找到一个新的多态性SSR标记。BC1F群中的所有耐氟个体均表现出与(733新×T6)F1相同的杂合型带型,而所有敏感个体带型与亲本733新一致,均为纯合型,说明家蚕耐氟性状是由位于第12连锁群上单个显性主效基因控制的。利用另一个群体BCM构建了耐氟基因的遗传连锁图。
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| 关 键 词： | 家蚕 耐氟基因 连锁 定位 微卫星标记 |
SSR Based Linkage and Mapping Analysis of Dominant Endurance to Fluoride Gene (Def) in the Silkworm, Bombyx mori |
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| BAI Hui-Chai,XU An-Ying,LI Mu-Wang,ZHAO Yun-Po,GUO Qiu-Hong,QIAN He-Ying,ZHANG Yue-Hua,ZHAO Yuan,HUANG Yong-Ping,GUO Xi-Jie.SSR Based Linkage and Mapping Analysis of Dominant Endurance to Fluoride Gene (Def) in the Silkworm, Bombyx mori[J].Acta Sericologica Sinica,2008,34(2):191-196. |
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| Authors: | BAI Hui-Chai XU An-Ying LI Mu-Wang ZHAO Yun-Po GUO Qiu-Hong QIAN He-Ying ZHANG Yue-Hua ZHAO Yuan HUANG Yong-Ping GUO Xi-Jie |
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| Abstract: | In the silkworm, the endurance to fluoride is controlled by a dominant gene, Def. Silkworm strain 733 xin is highly sensitive to fluoride (+Def/+Def), while T6 is endurable to fluoride (Def/Def). Owning to a lack of crossing over in females, reciprocal backcrossed F1 (BC1) progeny were used for linkage analysis and mapping of the Def gene based on the constructed SSR map. With the markers in 28 groups of Bombyx mori, the interest gene was found to be linked to 3 SSR markers in the 12th group. By screening other SSR site in the scaffolds which harbored the SSR markers in the 12th group of the SSR linkage map, a new SSR marker was found to be linked to Def gene. All the individuals which were endurable to fluoride in the BC1F showed heterozygous profile of (733 xin×T6) F1, and the larva with sensitive to fluoride hemolymph in BC1F showed the homozygous profile of the strain 733 xin. Using a reciprocal BC1M cross, we constructed a linkage map about Def gene. |
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| Keywords: | Bombyx mori Def gene Linkage Gene location Microsatellite marker |
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