| 对家蚕多星纹基因ms的SSR标记定位分析 |
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| 引用本文： | 张月华,张俊,李刚,徐安英,沈兴家,孙平江,钱荷英,苗雪霞,黄勇平.对家蚕多星纹基因ms的SSR标记定位分析[J].蚕业科学,2011,37(3). |
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| 作者姓名： | 张月华 张俊 李刚 徐安英 沈兴家 孙平江 钱荷英 苗雪霞 黄勇平 |
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| 作者单位： | 江苏科技大学,江苏镇江,212003;中国农业科学院蚕业研究所,江苏镇江,212018;江苏科技大学,江苏镇江,212003;中国农业科学院蚕业研究所,江苏镇江,212018;中国科学院上海生命科学研究院植物生理生态研究所,上海,200032 |
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| 基金项目： | 现代农业产业技术体系建设专项(蚕桑) |
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| 摘 要： | 对家蚕幼虫斑纹突变多星纹基因ms进行分子标记定位,有益于对多星纹性状的分子标记辅助选择和对该基因的定位克隆研究。利用家蚕雌性染色体在减数分裂过程中不发生交换的特点,采用幼虫斑纹为素斑(p)的家蚕品系C108,幼虫斑纹为多星纹(ms)的家蚕品系g02,组配正反交群体(g02×C108)F1♀×g02♂和g02♀×(g02×C108)F1♂,分别记作BC1F和BC1M,利用已经构建的家蚕SSR分子标记连锁图谱中第12连锁群上的18对SSR标记引物在亲本间进行多态性筛选。BC1F群体中的普通斑个体均表现出与(g02×C108)F1相同的杂合型带型;而所有多星纹个体的带型与亲本g02一致,为纯合型。结果筛选出S1206、S1208、S1210、C5553S3共4个与家蚕ms连锁的SSR标记。利用BC1M群体构建家蚕ms及其连锁的SSR标记遗传连锁图,连锁图的图距为34.5 cM,4个SSR标记及ms的排列次序为S1206—S1208—S1210—C5553S3—ms,ms位于34.5 cM处,与ms最近的标记为C5553S3,遗传距离为12.4 cM。依据该SSR标记遗传连锁图谱可对ms进一步精确定位。
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| 关 键 词： | 家蚕 幼虫斑纹 多星纹基因 基因定位 SSR标记 |
SSR Based Mapping Analysis of Multistar(ms) Gene in Silkworm,Bombyx mori |
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| ZHANG Yue-Hua,ZHANG Jun,LI Gang,XU An-Ying,SHEN Xing-Jia,SUN Ping-Jiang,QIAN He-Ying,MIAO Xue-Xia,HUANG Yong-Ping.SSR Based Mapping Analysis of Multistar(ms) Gene in Silkworm,Bombyx mori[J].Acta Sericologica Sinica,2011,37(3). |
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| Authors: | ZHANG Yue-Hua ZHANG Jun LI Gang XU An-Ying SHEN Xing-Jia SUN Ping-Jiang QIAN He-Ying MIAO Xue-Xia HUANG Yong-Ping |
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| Institution: | ZHANG Yue-Hua1,2 ZHANG Jun1 LI Gang1 XU An-Ying2* SHEN Xing-Jia1,2 SUN Ping-Jiang2 QIAN He-Ying2 MIAO Xue-Xia3 HUANG Yong-Ping3(1Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212003,China,2The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,3Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China) |
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| Abstract: | Gene mapping of stripes mutant multistar gene(ms) of silkworm(Bombyx mori) larvae is beneficial to molecular marker-assisted selection of silkworm multistar trait and to positional cloning studies of this gene.By utilizing the characteristic of non-crossing-over between chromosomes in female adult,silkworm strain g02 of which larva has multistar marking and C108 of which larva has plain marking were used to prepare reciprocal populations(g02×C108) F1♀×g02♂ and g02♀×(g02×C108) F1♂,designated as BC1F and BC1M.Based on the 12th linkage group of already established silkworm SSR molecular marker linkage map,18 pairs of SSR marker primers were used to conduct polymorphic screening among the parental populations.All larva individuals having normal marking in BC1F population showed identical heterozygous band pattern with(g02×C108) F1,while all larva individuals having multistar marking in BC1F population showed identical homozygous band pattern with the parental strain g02.As a result,4 SSR markers,namely S1206,S1208,S1210 and C5553S3,were found to link with silkworm ms gene.The reciprocal population BC1M was utilized to construct a genetic linkage map containing silkworm ms gene and its linked SSR markers.The obtained linkage map has a genetic distance of 34.5 cM.The 4 SSR markers and ms gene are arranged in the order of S1206—S1208—S1210—C5553S3—ms.ms is located at 34.5 cM.The marker closest to ms gene is C5553S3,with a genetic distance of 12.4 cM.Based on this SSR marker genetic linkage map,ms gene can be further mapped precisely. |
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| Keywords: | Bombyx mori Larval marking ms gene Gene mapping SSR marker |
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